EC Number |
Protein Variants |
Reference |
---|
2.4.3.4 | A151D |
the mutant shows significantly improved properties as compared to the native enzyme. The mutant displays overall best performance in the synthesis of the GM3 trisaccharide (alpha2,3-Neu5Ac-Lac) from lactose in a one-pot, two-enzyme cascade |
-, 759047 |
2.4.3.4 | C59S/C64S |
the mutant shows reduced activity compared to the wild type enzyme |
760184 |
2.4.3.4 | E271F |
the mutation does not affect alpha2-3-sialyltransferase activity |
723156 |
2.4.3.4 | E271F/R313Y |
the mutations do not affect alpha2-3-sialyltransferase activity |
723156 |
2.4.3.4 | H299A |
inactive |
760184 |
2.4.3.4 | H316A |
inactive |
760184 |
2.4.3.4 | L387A |
the mutant shows significantly improved properties as compared to the native enzyme |
-, 759047 |
2.4.3.4 | N147S |
the mutant shows strongly reduced activity compared to the wild type enzyme |
760184 |
2.4.3.4 | N170A |
the mutant shows strongly reduced activity compared to the wild type enzyme |
760184 |
2.4.3.4 | R313Y |
the mutation does not affect alpha2-3-sialyltransferase activity |
723156 |