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Literature summary for 2.4.3.4 extracted from

  • Sugiarto, G.; Lau, K.; Li, Y.; Khedri, Z.; Yu, H.; Le, D.T.; Chen, X.
    Decreasing the sialidase activity of multifunctional Pasteurella multocida alpha2-3-sialyltransferase 1 (PmST1) by site-directed mutagenesis (2011), Mol. Biosyst., 7, 3021-3027.
    View publication on PubMed
Search for more literature for 2.4.3.4

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 (DE3) cells Pasteurella multocida

Protein Variants

Protein Variants Comment Organism
E271F the mutation does not affect alpha2-3-sialyltransferase activity Pasteurella multocida
E271F/R313Y the mutations do not affect alpha2-3-sialyltransferase activity Pasteurella multocida
R313Y the mutation does not affect alpha2-3-sialyltransferase activity Pasteurella multocida

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.18
-
 CMP-N-acetylneuraminate mutant enzyme E271F, in Tris-HCl buffer (100 mM, pH 8.5), at 37°C Pasteurella multocida
0.34
-
 CMP-N-acetylneuraminate mutant enzyme E271F/R313Y, in Tris-HCl buffer (100 mM, pH 8.5), at 37°C Pasteurella multocida
0.44
-
 CMP-N-acetylneuraminate wild type enzyme, in Tris-HCl buffer (100 mM, pH 8.5), at 37°C Pasteurella multocida
0.54
-
 4-methylumbelliferyl 4-O-beta-D-galactosyl-beta-D-glucoside mutant enzyme E271F/R313Y, in Tris-HCl buffer (100 mM, pH 8.5), at 37°C Pasteurella multocida
0.62
-
 CMP-N-acetylneuraminate mutant enzyme R313Y, in Tris-HCl buffer (100 mM, pH 8.5), at 37°C Pasteurella multocida
0.67
-
 4-methylumbelliferyl 4-O-beta-D-galactosyl-beta-D-glucoside mutant enzyme R313Y, in Tris-HCl buffer (100 mM, pH 8.5), at 37°C Pasteurella multocida
0.71
-
 4-methylumbelliferyl 4-O-beta-D-galactosyl-beta-D-glucoside mutant enzyme E271F, in Tris-HCl buffer (100 mM, pH 8.5), at 37°C Pasteurella multocida
1.4
-
 4-methylumbelliferyl 4-O-beta-D-galactosyl-beta-D-glucoside wild type enzyme, in Tris-HCl buffer (100 mM, pH 8.5), at 37°C Pasteurella multocida

Organism

Organism UniProt Comment Textmining
Pasteurella multocida
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni2+-NTA column chromatography Pasteurella multocida

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
CMP-N-acetylneuraminate + 4-methylumbelliferyl 4-O-beta-D-galactosyl-beta-D-glucoside best substrate, about 37% conversion Pasteurella multocida CMP + 4-methylumbelliferyl alpha-N-acetylneuraminyl-(2->3)-beta-D-galactosyl-(1->4)-beta-D-glucoside
-
 ?
CMP-N-acetylneuraminate + lactose
-
 Pasteurella multocida CMP + NeuAcalpha(2->3)Galbeta(1->4)Glc
-
 ?

Synonyms

Synonyms Comment Organism
alpha2-3-sialyltransferase 1 multifunctional enzyme which has alpha2-6-sialyltransferase, alpha2-3-sialidase, and alpha2-3-trans-sialidase activities in addition to its major alpha2-3-sialyltransferase activity Pasteurella multocida
ST1
-
 Pasteurella multocida

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2 8 4-methylumbelliferyl 4-O-beta-D-galactosyl-beta-D-glucoside mutant enzyme E271F, in Tris-HCl buffer (100 mM, pH 8.5), at 37°C Pasteurella multocida
17
-
 4-methylumbelliferyl 4-O-beta-D-galactosyl-beta-D-glucoside mutant enzyme E271F/R313Y, in Tris-HCl buffer (100 mM, pH 8.5), at 37°C Pasteurella multocida
19
-
 CMP-N-acetylneuraminate mutant enzyme R313Y, in Tris-HCl buffer (100 mM, pH 8.5), at 37°C Pasteurella multocida
19
-
 4-methylumbelliferyl 4-O-beta-D-galactosyl-beta-D-glucoside mutant enzyme R313Y, in Tris-HCl buffer (100 mM, pH 8.5), at 37°C Pasteurella multocida
23
-
 CMP-N-acetylneuraminate mutant enzyme E271F/R313Y, in Tris-HCl buffer (100 mM, pH 8.5), at 37°C Pasteurella multocida
26
-
 CMP-N-acetylneuraminate mutant enzyme E271F, in Tris-HCl buffer (100 mM, pH 8.5), at 37°C Pasteurella multocida
32
-
 CMP-N-acetylneuraminate wild type enzyme, in Tris-HCl buffer (100 mM, pH 8.5), at 37°C Pasteurella multocida
47
-
 4-methylumbelliferyl 4-O-beta-D-galactosyl-beta-D-glucoside wild type enzyme, in Tris-HCl buffer (100 mM, pH 8.5), at 37°C Pasteurella multocida

pH Range

pH Minimum pH Maximum Comment Organism
6 10
-
 Pasteurella multocida

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
28
-
 4-methylumbelliferyl 4-O-beta-D-galactosyl-beta-D-glucoside mutant enzyme R313Y, in Tris-HCl buffer (100 mM, pH 8.5), at 37°C Pasteurella multocida
30
-
 CMP-N-acetylneuraminate mutant enzyme R313Y, in Tris-HCl buffer (100 mM, pH 8.5), at 37°C Pasteurella multocida
32
-
 4-methylumbelliferyl 4-O-beta-D-galactosyl-beta-D-glucoside mutant enzyme E271F/R313Y, in Tris-HCl buffer (100 mM, pH 8.5), at 37°C Pasteurella multocida
34
-
 4-methylumbelliferyl 4-O-beta-D-galactosyl-beta-D-glucoside wild type enzyme, in Tris-HCl buffer (100 mM, pH 8.5), at 37°C Pasteurella multocida
39
-
 4-methylumbelliferyl 4-O-beta-D-galactosyl-beta-D-glucoside mutant enzyme E271F, in Tris-HCl buffer (100 mM, pH 8.5), at 37°C Pasteurella multocida
69
-
 CMP-N-acetylneuraminate mutant enzyme E271F/R313Y, in Tris-HCl buffer (100 mM, pH 8.5), at 37°C Pasteurella multocida
73
-
 CMP-N-acetylneuraminate wild type enzyme, in Tris-HCl buffer (100 mM, pH 8.5), at 37°C Pasteurella multocida
140
-
 CMP-N-acetylneuraminate mutant enzyme E271F, in Tris-HCl buffer (100 mM, pH 8.5), at 37°C Pasteurella multocida