EC Number   |
Protein Variants |
Reference |
|---|
  2.1.1.B76 | D257G |
random mutagenesis, the mutant of POMT-7, POMT7-M1, has a novel regioselectivity, from large scale mutant screening, mutant D257G methylates the 3-hydroxyl group of flavonols in addition to 7-hydrdoxyl group |
713037 |
| 2.1.1.B76 | more |
combines engineered and optimization of enzyme expression and activity with cofacor production in recombinant Escherichia coli for improved biosynthesis of rhamnetin |
720210 |
| 2.1.1.B76 | more |
optimization of POMT7 expression and rhamnetin production in Escherichia coli, in addition, the S-adenosylmethionine biosynthesis pathway is engineered by coexpression of SAM synthetase, for optimal cofactor production, production of rhamnetin of up to 111 mg/l, overview |
720210 |
| 2.1.1.B76 | N257G |
the mutant methylates the 3-hydroxyl group of flavonols in addition to 7-hydrdoxyl group. The wild type POMT7 exhibits better activity toward kaempferol and quercetin than the mutant enzyme |
713037 |
