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Literature summary for 2.1.1.B76 extracted from

  • Sung, S.; Kim, B.; Ahn, J.
    Optimization of rhamnetin production in Escherichia coli (2011), J. Microbiol. Biotechnol., 21, 854-857.
    View publication on PubMed
Search for more literature for 2.1.1.B76

Application

Application Comment Organism
pharmacology the enzyme reaction product rhmanetin, produced from quercetin, inhibits the formation of beta-amyloid. Rhamnetin holds great promise for use in therapeutic application in neurodegenerative disease, method evaluation, overview Populus sp.
synthesis mass production of rhamnetin by recombinant Escherichia coli for medical use Populus sp.

Cloned(Commentary)

Cloned (Comment) Organism
functional POMT7 expression in Escherichia coli Populus sp.
gene POMT7, recombinant functional expression in Escherichia coli strain BL21(DE3), which is capable to transform quercetin into rhamnetin, coexpression with Escherichia coli SAM synthetase Populus sp.

Protein Variants

Protein Variants Comment Organism
additional information optimization of POMT7 expression and rhamnetin production in Escherichia coli, in addition, the S-adenosylmethionine biosynthesis pathway is engineered by coexpression of SAM synthetase, for optimal cofactor production, production of rhamnetin of up to 111 mg/l, overview Populus sp.
additional information combines engineered and optimization of enzyme expression and activity with cofacor production in recombinant Escherichia coli for improved biosynthesis of rhamnetin Populus sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + 3,3',4',5,7-pentahydroxyflavone Populus sp. i.e. quercetin S-adenosyl-L-homocysteine + 3,3',4',5-tetrahydroxy-7-methoxyflavone i.e. rhamnetin ?
S-adenosyl-L-methionine + quercetin Populus sp.
-
 S-adenosyl-L-homocysteine + 7-O-methylquercetin i.e. rhamnetin ?

Organism

Organism UniProt Comment Textmining
Populus sp.
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the O-methyltransferase from poplar transfers a methyl group to several flavonoids that contain a 7-hydroxyl group. POMT7 has a high affinity toward quercetin, and the reaction product rhamnetin Populus sp. ?
-
 ?
S-adenosyl-L-methionine + 3,3',4',5,7-pentahydroxyflavone i.e. quercetin Populus sp. S-adenosyl-L-homocysteine + 3,3',4',5-tetrahydroxy-7-methoxyflavone i.e. rhamnetin ?
S-adenosyl-L-methionine + quercetin
-
 Populus sp. S-adenosyl-L-homocysteine + 7-O-methylquercetin i.e. rhamnetin ?
S-adenosyl-L-methionine + quercetin POMT7 has a high affinity toward quercetin Populus sp. S-adenosyl-L-homocysteine + 7-O-methylquercetin i.e. rhamnetin ?

Synonyms

Synonyms Comment Organism
flavone 7-O-methyltransferase
-
 Populus sp.
POMT7
-
 Populus sp.

Cofactor

Cofactor Comment Organism Structure
S-adenosyl-L-methionine
-
 Populus sp.

General Information

General Information Comment Organism
additional information rhamnetin inhibits the formation of beta-amyloid Populus sp.