Application | Comment | Organism |
---|---|---|
pharmacology | the enzyme reaction product rhmanetin, produced from quercetin, inhibits the formation of beta-amyloid. Rhamnetin holds great promise for use in therapeutic application in neurodegenerative disease, method evaluation, overview | Populus sp. |
synthesis | mass production of rhamnetin by recombinant Escherichia coli for medical use | Populus sp. |
Cloned (Comment) | Organism |
---|---|
functional POMT7 expression in Escherichia coli | Populus sp. |
gene POMT7, recombinant functional expression in Escherichia coli strain BL21(DE3), which is capable to transform quercetin into rhamnetin, coexpression with Escherichia coli SAM synthetase | Populus sp. |
Protein Variants | Comment | Organism |
---|---|---|
additional information | optimization of POMT7 expression and rhamnetin production in Escherichia coli, in addition, the S-adenosylmethionine biosynthesis pathway is engineered by coexpression of SAM synthetase, for optimal cofactor production, production of rhamnetin of up to 111 mg/l, overview | Populus sp. |
additional information | combines engineered and optimization of enzyme expression and activity with cofacor production in recombinant Escherichia coli for improved biosynthesis of rhamnetin | Populus sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + 3,3',4',5,7-pentahydroxyflavone | Populus sp. | i.e. quercetin | S-adenosyl-L-homocysteine + 3,3',4',5-tetrahydroxy-7-methoxyflavone | i.e. rhamnetin | ? | |
S-adenosyl-L-methionine + quercetin | Populus sp. | - |
S-adenosyl-L-homocysteine + 7-O-methylquercetin | i.e. rhamnetin | ? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Populus sp. | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the O-methyltransferase from poplar transfers a methyl group to several flavonoids that contain a 7-hydroxyl group. POMT7 has a high affinity toward quercetin, and the reaction product rhamnetin | Populus sp. | ? | - |
? | |
S-adenosyl-L-methionine + 3,3',4',5,7-pentahydroxyflavone | i.e. quercetin | Populus sp. | S-adenosyl-L-homocysteine + 3,3',4',5-tetrahydroxy-7-methoxyflavone | i.e. rhamnetin | ? | |
S-adenosyl-L-methionine + quercetin | - |
Populus sp. | S-adenosyl-L-homocysteine + 7-O-methylquercetin | i.e. rhamnetin | ? | |
S-adenosyl-L-methionine + quercetin | POMT7 has a high affinity toward quercetin | Populus sp. | S-adenosyl-L-homocysteine + 7-O-methylquercetin | i.e. rhamnetin | ? |
Synonyms | Comment | Organism |
---|---|---|
flavone 7-O-methyltransferase | - |
Populus sp. |
POMT7 | - |
Populus sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
S-adenosyl-L-methionine | - |
Populus sp. |
General Information | Comment | Organism |
---|---|---|
additional information | rhamnetin inhibits the formation of beta-amyloid | Populus sp. |