EC Number |
---|
4.6.1.1 | - |
4.6.1.1 | 2.8 A and 2.9 A resolutions, crystal structures of the complex between stimulatory G protein alpha subunits guanosine 5'-(gamma-thio)triphosphate and the catalytic C1 and C2 domains from type V and type II adenylate cyclase, bound to forskolin and either 2'(3')-O-(N-methylanthraniloyl)-guanosine 5' triphosphate Mg2+ or 2'(3')-O-(N-methylanthraniloyl)-guanosine 5' triphosphate Mn2+ |
4.6.1.1 | 3.2 A resolution |
4.6.1.1 | 3.3 A resolution active form of the catalytic domain Rv1264, 2.3 A resolution inhibited form |
4.6.1.1 | at 1.9 A resolution, crystals belong to space group P212121, eight highly conserved ionic residues, E10, E12, K14, R63, K76, K111, D126, and E136 lie in the barrel core and form the likely binding sites for substrate and divalent cations. A phosphate ion bound to R63, K76, K111, and R113 near the center of the conserved cluster. AC-IV active site is relatively enriched in glutamate and features an ExE motif as its most conserved element |
4.6.1.1 | by the hanging-drop, vapour-diffusion method at 18°C, to 1.6 A resolution, crystal structure of the histidine-tagged auto-inhibitory domain Rv1264N shows the protein in a tight, disk-shaped dimer, formed around a helical bundle, and involving a protein chain crossover |
4.6.1.1 | by vapour diffusion-hanging drop method, amino-terminal His-tagged construct, from which the tag was removed by thrombin, crystallized in a triclinic form diffracting to 1.9 A resolution, with one dimer per asymmetric unit and unit-cell parameters a = 33.5, b = 35.5, c = 71.8 A, alpha = 88.7, beta = 82.5, gamma = 65.5°. Non-His tagged native construct crystallized in space group P212121 with unit-cell parameters a = 56.8, b = 118.6, c = 144.5 A, to 2.9 A resolution and probably having two dimers per asymmetric unit |
4.6.1.1 | complex with 2-hydroxyestradiol and alpha/beta-methylene-ATP, 20-3 A resolution |
4.6.1.1 | crystal structure analysis |
4.6.1.1 | crystal structure of native soluble AC and of soluble AC in complex with the competitiv substrate analogs beta-L-2',3'-dideoxyadenosine 5'-triphiosphate and adenosine 5'-(alpha-thio)-triphosphate at 2.8-3.0 A |