EC Number   |
|---|
    2.5.1.18 | AtuGSTH1-1 in complex with inhibitor S-(4-nitrobenzyl)-glutathione, hanging drop vapor diffusion method, mixing of 0.002 ml of 4.85 mg/ml protein in 15 mM Tris-HCl, pH 7.0, and 10 mM S-(4-nitrobenzyl)-glutathione, with 0.002 ml of well solution 1.4 M Na/K phosphate, pH 8.3, equilibration against 0.8 ml well solution, 16°C, X-ray diffraction structure determination and analysis at 1.4 A resolution |
| 2.5.1.18 | complexed with glutathione or its analogues |
| 2.5.1.18 | crystals belong to the triclinic space group P1, with unit-cell parameters a = 72.7, b = 74.0, c = 88.6 A, alpha = 79.1°, beta = 80.1°, gamma = 81.5°, likely contains four homodimers per asymmetric unit |
| 2.5.1.18 | dmGSTD10 in apo- and glutathione-bound form, and dmGSTD1, hanging drop vapor diffusion method, mixing of 0.002 ml of 10 mg/ml protein in 50 mM Tris-HCl, pH 7.5, with 0.002 ml of reservoir solution16 and 23°C, X-ray diffraction structure determination and analysis, attempts to crystallize dmGSTD1 apo-form are unsuccessful due to its affinity toward glutathione ligand |
| 2.5.1.18 | enzyme in complex with GSH is determined at 2.4 A |
| 2.5.1.18 | enzyme in complex with substrates glutathione or 1-chloro-2,4-dinitrobenzene, sitting-drop vapour-diffusion method, 0.004 ml of protein solution, containing 20 mM Tris-HCl, pH 7.9, 200 mM NaCl, 2 mM DTT, is mixed with 0.004 ml of reservoir solution containing 6% Tacsimate, pH 8.0, and 26% w/v PEG 3350, for cocrystallization, the protein solution is mixed with glutathione at a molar ratio of 1:1 and with 1-chloro-2,4-dinitrobenzene at a molar ratio of 1:5. The glutathionecomplex crystals from 7% Tacsimate, pH 8.0, 24% w/v PEG 3350 and the CDNB-complex crystals from 8.5% Tacsimate, pH 8.0, 25% w/v PEG 335. Equilibration over 0.5 ml reservoir solution, 22°C, 3 days, X-ray diffraction structure determination and analysis at 2.2 A and 2.0 A resolution, respectively |
| 2.5.1.18 | enzyme in GSH-free and -bound forms, using 20% (w/v) PEG8000, 100 mM HEPES-HCl (pH 7.5), 200 mM ammonium sulfate, and 20% (v/v) 2-propanol at 4°C |
| 2.5.1.18 | hanging drop vapor diffusion method |
| 2.5.1.18 | hanging drop vapor diffusion method, using 0.1 M HEPES pH 7.5, 20% (w/v) PEG 4000, 10% (v/v) 2-propanol, 2 mM GSH, 10 mM dithiothreitol |
| 2.5.1.18 | hanging drop vapor diffusion method, using 0.1 M Tris-HCl pH 7.5, 25% (w/v) PEG 4000, 2 mM GSH, and 10 mM dithiothreitol |
