EC Number   |
pH Stability |
pH Stability Maximum |
Reference |
|---|
    1.7.3.3 | -999 |
- |
uricase activity in 50 h culture broth with pH values of 5.5 and 6.0 decreases more rapidly than that in cultures with pH values of 6.5 and 7.0, at pH 5.5, about 78% of initial uricase activity is lost within 25 h, under the same conditions, more than 85% of initial uricase activity remains in culture broth of pH 6.5 and 7.0, uricase activity in 66 h culture broth with pH 7.0 degrades much more rapidly than that in samples from 50 h culture, while for pH 6.5, the uricase is still stable, loss of uricase activity is caused by the degradation in acidic environment by proteases secreted by the host cells or releases from host cell lyses, low pH may cause instability of uricase |
695795 |
| 1.7.3.3 | 6 |
- |
25°C, 18 h, about 60% loss of activity |
701416 |
| 1.7.3.3 | 6 |
- |
45°C, 30 min, 40% loss of activity |
394146 |
| 1.7.3.3 | 6 |
10 |
the purified enzyme remains stable over a wide range of pH between 6.0-10.0 for 2 h |
764830 |
| 1.7.3.3 | 6 |
11 |
35°C, 1 h, stable |
394144 |
| 1.7.3.3 | 6.5 |
10.5 |
22°C, 30 min, immobilized enzyme, stable |
394156 |
| 1.7.3.3 | 7 |
- |
UOX is deactivated at different protein concentrations at 45°C in 20 mM phosphate containing 0.15 M NaCl, 0.01 mg/ml UOX is deactivated much faster than its counterparts at concentrations of 0.1 and 1.0 mg/ml |
700631 |
| 1.7.3.3 | 7 |
9.5 |
in case of uricase entrapped in lipid vesicles, the remaining activity keeps more than 90% during the pH of 7.0-9.5, and the maximum remaining activity is 98.04% at pH 8.0 when incubated at 40°C or 40 min. For the free uricase, the maximum remaining activity is 86.59% at pH 8.5 when incubated at 40°C or 40 min |
712197 |
| 1.7.3.3 | 7 |
10 |
- |
743753 |
| 1.7.3.3 | 7 |
10 |
purified enzyme, 25°C, 18 h, stable |
701416 |
