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Literature summary for 1.7.3.3 extracted from
- Purification and properties of urate oxidase from Streptomyces cyanogenus (1981), J. Biochem., 89, 1769-1776.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-Hydroxypurine | - |
Streptomyces cyanogenus |  |
| CN- | - |
Streptomyces cyanogenus | |
| iodoacetate | low effect | Streptomyces cyanogenus | |
| p-chloromercuribenzoate | slight effect | Streptomyces cyanogenus | |
| Urate | not | Streptomyces cyanogenus | |
| xanthine | - |
Streptomyces cyanogenus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.099 | - |
uric acid | - |
Streptomyces cyanogenus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cell associated | - |
Streptomyces cyanogenus | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| copper | enzyme contains 0.15 mol of copper | Streptomyces cyanogenus | |
| Iron | enzyme contains less than 0.05 mol of iron | Streptomyces cyanogenus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 32000 | - |
alpha3, 3 * 32000, SDS-PAGE | Streptomyces cyanogenus |
| 109000 | - |
gel filtration | Streptomyces cyanogenus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| urate + O2 + H2O | Streptomyces cyanogenus | - |
5-hydroxyisourate + H2O2 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Streptomyces cyanogenus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Streptomyces cyanogenus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 12.3 | - |
- |
Streptomyces cyanogenus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, 20 mM phosphate buffer, pH 7.8, for at least 1 month | Streptomyces cyanogenus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| urate + O2 + H2O | - |
Streptomyces cyanogenus | 5-hydroxyisourate + H2O2 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| trimer | alpha3, 3 * 32000, SDS-PAGE | Streptomyces cyanogenus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | - |
- |
Streptomyces cyanogenus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
pH 7.8, 10 min, stable below | Streptomyces cyanogenus |
| 60 | - |
pH 7.8, 10 min, 50% loss of activity | Streptomyces cyanogenus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | 8.5 | borate and phosphate buffer | Streptomyces cyanogenus |
| 9.5 | - |
glycine buffer | Streptomyces cyanogenus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 11 | 35°C, 1 h, stable | Streptomyces cyanogenus |
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