EC Number   |
Turnover Number Minimum [1/s] |
Turnover Number Maximum [1/s] |
Substrate |
Reference |
|---|
   6.5.1.1 | -999 |
- |
more |
steady state experiments with a nicked substrate containing juxtaposed dC and 5'-phosphorylated dT deoxynucleotides yield kcat and kcat/Km values of 0.4/sec and 150/microM/sec, respectively. Under identical reaction conditions, turnover of an adenylylated version of this substrate yield kcat and kcat/Km values of 0.64/sec and 240 microM/sec |
727896 |
| 6.5.1.1 | -999 |
- |
more |
turnover numbers as a function of 3'-base pair identity |
661207 |
| 6.5.1.1 | 0.0006 |
- |
methylenebisphosphonate |
in 50 mM HEPES/KOH (pH 7.2), 1 mM dithiothreitol, 5 mM MgCl2, at 30°C |
690851 |
| 6.5.1.1 | 0.0008 |
- |
geranyl diphosphate |
- |
707449 |
| 6.5.1.1 | 0.0018 |
- |
(deoxyribonucleotide)20 |
25°C, pH 7.5 |
650253 |
| 6.5.1.1 | 0.0023 |
- |
nicked DNA |
37°C, pH 8.5 |
675877 |
| 6.5.1.1 | 0.0026 |
- |
ATP |
30°C |
652248 |
| 6.5.1.1 | 0.0028 |
- |
farnesyl diphosphate |
- |
707449 |
| 6.5.1.1 | 0.0041 |
- |
clodronate |
in 50 mM HEPES/KOH (pH 7.2), 1 mM dithiothreitol, 5 mM MgCl2, at 30°C |
690851 |
| 6.5.1.1 | 0.0062 |
- |
tripolyphosphate |
in 50 mM HEPES/KOH (pH 7.2), 1 mM dithiothreitol, 5 mM MgCl2, at 30°C |
690851 |
