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Literature summary for 6.5.1.1 extracted from

  • Ranalli, T.A.; DeMott, M.S.; Bambara, R.A.
    Mechanism underlying replication protein A stimulation of DNA ligase I (2002), J. Biol. Chem., 277, 1719-1727.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
replication protein A approx. 15fold stimulation of DNA ligase I Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
-
Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0000034
-
ATP 30°C Homo sapiens
0.0000049
-
ATP 30°C, in the presence of 0.0000125 mM replication protein A Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m Homo sapiens
-
AMP + diphosphate + (deoxyribonucleotide)m+n
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant DNA ligase I Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m
-
Homo sapiens AMP + diphosphate + (deoxyribonucleotide)m+n
-
?

Synonyms

Synonyms Comment Organism
DNA ligase I
-
Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0026
-
ATP 30°C Homo sapiens
0.012
-
ATP 30°C, in the presence of 0.0000125 mM replication protein A Homo sapiens