EC Number |
Temperature Stability Minimum [°C] |
Temperature Stability Maximum [°C] |
Reference |
---|
3.6.1.1 | -999 |
- |
residue Cys116 is involved in thermostability |
684755 |
3.6.1.1 | -999 |
- |
structural basis for the high thermostability |
667993 |
3.6.1.1 | 0 |
- |
stable above 0.05 mM in Tris-HCl, Mg(CH3COO)2, 0.5 mM DTT, 2 h |
209775 |
3.6.1.1 | 0 |
25 |
stable to freezing, 0°C and thawing 25° |
209768 |
3.6.1.1 | 20 |
- |
purified enzyme, quite stable |
688745 |
3.6.1.1 | 20 |
- |
stable above 0.05 mM in Tris-HCl, Mg(CH3COO)2, 0.5 mM DTT, 2 h |
209775 |
3.6.1.1 | 25 |
- |
the enzyme remains active for 7 days |
728748 |
3.6.1.1 | 30 |
- |
60 min, purified enzyme, 54% remaining activity |
688745 |
3.6.1.1 | 35 |
- |
denaturation decreases with dithiothreitol |
209785 |
3.6.1.1 | 37 |
- |
15 min, the mutant enzyme loses 70% activity in absence of Mg2+, half-life is 4.2 min |
684755 |