Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.6.1.1 extracted from

  • Hachimori, A.; Fujii, T.; Ohki, K.; IIisuka, E.
    Purification and properties of inorganic pyrophosphatase from porcine brain (1983), J. Biochem., 93, 257-264.
    View publication on PubMed

General Stability

General Stability Organism
stability enhanced by bovine serum albumin or calmodulin Sus scrofa

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ 50% inhibition at 0.1 mM, no effect at physiological concentrations Sus scrofa
Cd2+
-
Sus scrofa
Cu2+
-
Sus scrofa
Mn2+
-
Sus scrofa
NaF
-
Sus scrofa

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ no other divalent cations can substitute for Mg2+ Sus scrofa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
33000
-
2 * 33000, SDS-PAGE Sus scrofa
62000
-
gel filtration Sus scrofa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
diphosphate + H2O Sus scrofa
-
2 phosphate
-
?

Organism

Organism UniProt Comment Textmining
Sus scrofa
-
pig
-

Purification (Commentary)

Purification (Comment) Organism
-
Sus scrofa

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
Sus scrofa
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1986
-
-
Sus scrofa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
diphosphate + H2O
-
Sus scrofa 2 phosphate
-
?

Subunits

Subunits Comment Organism
dimer 2 * 33000, SDS-PAGE Sus scrofa

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
-
-
stable above 0.05 mM in Tris-HCl, Mg(CH3COO)2, 0.5 mM DTT, 2 h Sus scrofa
20
-
stable above 0.05 mM in Tris-HCl, Mg(CH3COO)2, 0.5 mM DTT, 2 h Sus scrofa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.3
-
-
Sus scrofa

pH Range

pH Minimum pH Maximum Comment Organism
5.5 10
-
Sus scrofa