EC Number   |
Temperature Stability Minimum [°C] |
Temperature Stability Maximum [°C] |
Reference |
|---|
   3.4.21.62 | -999 |
- |
Ca2+ stabilizes the enzyme upon heat treatment |
29478 |
| 3.4.21.62 | -999 |
- |
requirement of Ca2+ ions for the hyperthermostability of Tk-subtilisin.The Ca1, Ca6, and Ca7 ions, especially the Ca1 ion, contribute to the hyperthermostabilization of Tk-subtilisin |
726988 |
| 3.4.21.62 | -999 |
- |
subtilisin Novo immobilized on porous glass with the aid of hexamethylene diisocyanate is stable in a wider range of temperature than the native enzyme |
29480 |
| 3.4.21.62 | -999 |
- |
the high structural similarity between Bacillus sphaericus Sph and S41 and their identical calcium-binding mode, indicates that the calcium-loaded state is not responsible for the cold adaptation of S41 |
710507 |
| 3.4.21.62 | -999 |
- |
thermostability profiles of purified wild-type enzyme, and mutants N253D, Q256E, and N253D/Q256E, overview |
731201 |
| 3.4.21.62 | 22 |
45 |
presence of Ca2+ |
667756 |
| 3.4.21.62 | 30 |
- |
purified recombinant enzyme, 60 min, 70% activity remaining |
752524 |
| 3.4.21.62 | 30 |
40 |
pH 10.5, stable for 2 h |
669738 |
| 3.4.21.62 | 37 |
- |
relatively unstable when incubated at 37°C for 18 h (80% reduction) |
718443 |
| 3.4.21.62 | 40 |
- |
purified recombinant enzyme, 45 h, 50% activity remaining, 90% remaning after 10 h |
754435 |
