EC Number   |
Temperature Stability Minimum [°C] |
Temperature Stability Maximum [°C] |
Reference |
|---|
   3.2.1.113 | -999 |
- |
mannosidase IA and B differ in thermostability |
135998 |
| 3.2.1.113 | 20 |
30 |
isoform ManIA2 shows more than 75% activity at 30°C in the presence of Ca2+, and more than 75% activity at 20°C in the absence of Ca2+ |
715684 |
| 3.2.1.113 | 25 |
- |
isozymes MANIa and MANIb are relatively stable at lower temperature and still retain 50% of their original activities after incubation at 25°C for 1 h and show a significant decrease of activities at higher temperatures with 10% activity at 30°C |
703909 |
| 3.2.1.113 | 37 |
- |
24 h, in 0.1 M sodium acetate buffer, pH 5.8, 30% loss of activity |
135998 |
| 3.2.1.113 | 40 |
- |
isoform ManIC shows more than 75% activity at 40°C in the absence and presence of Ca2+ |
715684 |
| 3.2.1.113 | 50 |
- |
90 min, about 60-70% loss of activity |
646570 |
| 3.2.1.113 | 50 |
- |
mannosidase IB less stable than IA |
135998 |
| 3.2.1.113 | 50 |
- |
Tm-value of mutant enzyme C443G, C334A, C363A and C443S |
664373 |
| 3.2.1.113 | 52 |
- |
Tm-value of mutant enzyme C443A |
664373 |
| 3.2.1.113 | 53 |
- |
Tm-value of mutant enzyme C443T |
664373 |
