EC Number |
Temperature Stability Minimum [°C] |
Temperature Stability Maximum [°C] |
Reference |
---|
1.13.11.52 | -999 |
- |
L-Trp stabilizes against heat inactivation |
395455 |
1.13.11.52 | -20 |
- |
losing 70% activity after 24 h, addition of 5 mg/ml BSA maintaines 80% activity after 5 days |
666239 |
1.13.11.52 | 4 |
- |
maintaines 70% activity with addition of 2 mg/ml catalase for 4 days |
666239 |
1.13.11.52 | 48 |
- |
complete denaturation of IDO2, partially of IDO1 |
723923 |
1.13.11.52 | 48 |
60 |
the melting temperature of the denaturation process for recombinant isozyme IDO1 is 60°C, with a possible second intermediate at 48°C, recombinant isozyme IDO2 denatures in a single step, with a melting temperature at 48°C |
710831 |
1.13.11.52 | 55 |
- |
pH 6.0, 5 min, 43% loss of dioxygenase activity on L-Trp and D-Trp, 55% loss ofter 10 min, 70% loss ofter 20 min |
395462 |
1.13.11.52 | 60 |
- |
complete denaturation of IDO1 |
723923 |
1.13.11.52 | 70 |
- |
30 min, stable in presence of 5 mM L-Trp |
395455 |
1.13.11.52 | 75 |
- |
30 min, about 5% loss of activity in presence of 5 mM L-Trp |
395455 |
1.13.11.52 | 80 |
- |
30 min, about 35% loss of activity in presence of 5 mM L-Trp |
395455 |