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EC Number	Temperature Optimum [�C]	Temperature Optimum Maximum [�C]	Commentary	Reference
6.5.1.1	4	-	ligation of cohesive ends	1910
6.5.1.1	8	-	-	662058
6.5.1.1	10	16	when polyethylene glycol is not present	1916
6.5.1.1	16	-	assay at	727896
6.5.1.1	22	-	assay at	727917
6.5.1.1	25	-	assay at	728407
6.5.1.1	25	-	blunt end ligation, 16mer or longer	1909
6.5.1.1	25	-	joining of the blunt ends of duplex structures 16 nucleotides or longer	1909
6.5.1.1	25	30	-	1922
6.5.1.1	37	-	-	675877, 716154

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Release 2023.1 (January 2023)