KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000001 | - |
(single-stranded DNA splinted by RNA)m | value below, pH 7.4, 25°C | Chlorella virus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | increases activity | Chlorella virus | |
NaCl | rate of ligation is inhibited by concentrations of more than 100 mM NaCl | Chlorella virus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Chlorella virus | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + (single-stranded DNA splinted by RNA)m + (single-stranded DNA splinted by RNA)n | - |
Chlorella virus | AMP + diphosphate + (single-stranded DNA splinted by RNA)m+n | - |
? | |
additional information | abortive adenylylation is suppressed at low ATP concentrations (below 100 mM) and pH higher than 8, leading to increased product yields. The ligation reaction is rapid for a broad range of substrate sequences, but is relatively slower for substrates with a 5'-phosphorylated dC or dG residue on the 3' side of the ligation junction | Chlorella virus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
DNA ligase | - |
Chlorella virus |
PBCV-1 DNA ligase | - |
Chlorella virus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Chlorella virus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.008 | - |
(single-stranded DNA splinted by RNA)m | pH 7.4, 25°C | Chlorella virus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Chlorella virus |