EC Number   |
|---|
    6.1.1.4 | - |
| 6.1.1.4 | 2 genes leuS and leuS', overexpression in Escherichia coli BL21(DE3) |
| 6.1.1.4 | DNA fragment encoding the fusion protein of Escherichia coli CP1 domain with Aquifex aeolicus beta-subunit and CP1 Escherichia coli beta-like polypeptide are inserted into pET30a by a two step procedure |
| 6.1.1.4 | Escherichia coli BL21-Codon Plus (DE3) cells are co-transformed with plasmid pSBETH6-lrsa and pET-15b-lrsb to produce alphabeta-LeuRS with an N-terminal His6 tag |
| 6.1.1.4 | expressed in Escherichia coli as a His-tagged fusion protein |
| 6.1.1.4 | expressed in Escherichia coli BL21(DE3) cells |
| 6.1.1.4 | expression and assembly of the 63 and 34 kDa peptides in Escherichia coli strain KL231 cannot complement the deficient temperature-sensitive strain, while expression of peptide fragments derived by cleavage of other peptide bonds leads to assembly of a functional enzyme |
| 6.1.1.4 | expression in Escherichia coli |
| 6.1.1.4 | expression in Escherichia coli strain KL231 cells |
| 6.1.1.4 | expression of alpha subunit and beta subunit in Escherichia coli |
