EC Number   |
|---|
    6.1.1.20 | - |
| 6.1.1.20 | complementatiom of a growth mutant defective in phenylalanylation in vivo by wild-type full-length PheRS from Saccharomyces cerevisiae, the full-length Escherichia coli enzyme A294G, and the truncated mutant PheRSDELTAB2, overexpression of full-length mutant A294G and truncated mutant PheRSDELTAB2A294G in strain XL-1 Blue |
| 6.1.1.20 | DNA and amino acid sequence determination of the alpha- and beta-subunit and analysis, expression in COS-7 cells |
| 6.1.1.20 | DNA and amino acid sequence determination, individual cloning and coexpression of His-tagged alpha- and beta-subunits in Escherichia coli resulting in 3 different types of (alphabeta)2 heterodimers with the His-tag at the N-terminus, N-terminally His-tagged beta-subunit renders the enzyme inactive, overexpression of nontagged alpha- and beta-subunits in Escherichia coli |
| 6.1.1.20 | DNA sequence determination and analysis |
| 6.1.1.20 | Escherichia coli BL21-RIL/pET16b produces His6-tagged mtPheRS encoded by the MSF1 gene, the ctPheRS alpha and beta subunits, encoded by the FRS1 and FRS2 genes, respectively, are expressed in tandem from pQE31-FRS-sc in Escherichia coli BL21-RIL cells |
| 6.1.1.20 | Escherichia coli pET21c+ encoding human mtPheRS, FARS2, produces mature His6-tagged wild-type PheRS |
| 6.1.1.20 | Escherichia coli strain BL21(pArgU218)/pET21c-PheRS expresses C-terminal His6-tagged mtPheRS, RosettaDE3 cells containing pRARE plasmids encoding tRNAs for rare codons are transformed with the mutant mtPheRS plasmid constructs |
| 6.1.1.20 | expressed in Escherichia coli |
| 6.1.1.20 | expressed in Escherichia coli BL21(DE3) cells |
