EC Number   |
|---|
    6.1.1.2 | - |
| 6.1.1.2 | cDNA fragments of human full-length TrpRS, aa 1-471, mini TrpRS, aa 48-471, and T1 TrpRS, aa 71-471, are separately cloned into the pET20b vector for expression in Escherichia coli BL21DE3 cells |
| 6.1.1.2 | determination of regulation involved promoter sequences |
| 6.1.1.2 | DNA and amino acid sequence determination and anaylsis, genetic structure, aaRS domain organization and sequence conservation and the sense/antisense coding hypothesis, overview, expression of FLAG-tagged wild-type and mutant enzymes and isolated catalytic domains in Escherichia coli strain BL21(DE3) inclusion bodies |
| 6.1.1.2 | DNA sequence analysis, expression of wild-type enzyme and a mutant enzyme, truncated in the CP2 domain, in Escherichia coli DH5alpha as fusion proteins with glutathione S-transferase |
| 6.1.1.2 | expressed in Escherichia coli |
| 6.1.1.2 | expressed in Escherichia coli B834 cells |
| 6.1.1.2 | expressed in Escherichia coli BL21(DE3)pLysS cells |
| 6.1.1.2 | expressed in Hepa 1-6 cells |
| 6.1.1.2 | expression in Escherichia coli |
