Information on EC 6.1.1.2 - tryptophan-tRNA ligase

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The expected taxonomic range for this enzyme is: Bacteria, Archaea, Eukaryota

EC NUMBER
COMMENTARY hide
6.1.1.2
-
RECOMMENDED NAME
GeneOntology No.
tryptophan-tRNA ligase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
ATP + L-tryptophan + tRNATrp = AMP + diphosphate + L-tryptophyl-tRNATrp
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Aminoacylation
esterification
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Aminoacyl-tRNA biosynthesis
-
-
tRNA charging
-
-
tryptophan metabolism
-
-
SYSTEMATIC NAME
IUBMB Comments
L-tryptophan:tRNATrp ligase (AMP-forming)
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CAS REGISTRY NUMBER
COMMENTARY hide
9023-44-3
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
gene trpS
-
-
Manually annotated by BRENDA team
-
SwissProt
Manually annotated by BRENDA team
-
SwissProt
Manually annotated by BRENDA team
contains a single TrpRS gene
UniProt
Manually annotated by BRENDA team
single copy gene
SwissProt
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
Balb/c mice
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
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-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
gene trpS
-
-
Manually annotated by BRENDA team
purified enzyme
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-
Manually annotated by BRENDA team
strain ATCC 12228; strains ATCC 12228 and ATCC 35984
SwissProt
Manually annotated by BRENDA team
gene trpRS1, an auxiliary, antibiotic-resistant tryptophanyl-tRNA synthetase gene
UniProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
evolution
physiological function
additional information
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + 2-azatryptophan + tRNATrp
AMP + diphosphate + 2-azatryptophyl-tRNATrp
show the reaction diagram
-
-
-
-
?
ATP + 4,5,6,7-tetrafluorotryptophan + tRNATrp
AMP + diphosphate + 4,5,6,7-tetrafluorotryptophyl-tRNATrp
show the reaction diagram
-
-
-
-
?
ATP + 4-aminotryptophan + tRNATrp
AMP + diphosphate + 4-aminotryptophyl-tRNATrp
show the reaction diagram
-
-
-
-
?
ATP + 4-fluorotryptophan + tRNATrp
AMP + diphosphate + 4-fluorotryptophyl-tRNATrp
show the reaction diagram
-
-
-
-
?
ATP + 4-hydroxytryptophan + tRNATrp
AMP + diphosphate + 4-hydroxytryptophyl-tRNATrp
show the reaction diagram
-
-
-
-
?
ATP + 4-methyltryptophan + tRNATrp
AMP + diphosphate + 4-methyltryptophyl-tRNATrp
show the reaction diagram
-
-
-
-
?
ATP + 5-aminotryptophan + tRNATrp
AMP + diphosphate + 5-aminotryptophyl-tRNATrp
show the reaction diagram
-
-
-
-
?
ATP + 5-bromotryptophan + tRNATrp
AMP + diphosphate + 5-bromotryptophyl-tRNATrp
show the reaction diagram
-
-
-
-
?
ATP + 5-fluorotryptophan + tRNATrp
AMP + diphosphate + 5-fluorotryptophyl-tRNATrp
show the reaction diagram
-
-
-
-
?
ATP + 5-hydroxy-L-tryptophan + tRNATrp
AMP + diphosphate + 5-hydroxy-L-tryptophan-tRNATrp
show the reaction diagram
-
mutant enzyme V144P selectively aminoacylates the cognate mutant opal suppressor tRNATrp(UCA) with 5-hydroxy-L-tryptophan and not with any endogenous amino acid
-
-
?
ATP + 5-hydroxytryptophan + tRNATrp
AMP + diphosphate + 5-hydroxytryptophyl-tRNATrp
show the reaction diagram
-
-
-
-
?
ATP + 5-iodotryptophan + tRNATrp
AMP + diphosphate + 5-iodotryptophyl-tRNATrp
show the reaction diagram
-
-
-
-
?
ATP + 5-methoxytryptophan + tRNATrp
AMP + diphosphate + 5-methoxytryptophyl-tRNATrp
show the reaction diagram
-
-
-
-
?
ATP + 5-methyltryptophan + tRNATrp
AMP + diphosphate + 5-methyltryptophyl-tRNATrp
show the reaction diagram
-
-
-
-
?
ATP + 6-aminotryptophan + tRNATrp
AMP + diphosphate + 6-aminotryptophyl-tRNATrp
show the reaction diagram
-
-
-
-
?
ATP + 6-fluorotryptophan + tRNATrp
AMP + diphosphate + 6-fluorotryptophyl-tRNATrp
show the reaction diagram
-
-
-
-
?
ATP + 6-methyltryptophan + tRNATrp
AMP + diphosphate + 6-methyltryptophyl-tRNATrp
show the reaction diagram
-
-
-
-
?
ATP + 7-aminotryptophan + tRNATrp
AMP + diphosphate + 7-aminotryptophyl-tRNATrp
show the reaction diagram
-
-
-
-
?
ATP + 7-azatryptophan + tRNATrp
AMP + diphosphate + 7-azatryptophyl-tRNATrp
show the reaction diagram
-
-
-
-
?
ATP + 7-methyltryptophan + tRNATrp
AMP + diphosphate + 7-methyltryptophyl-tRNATrp
show the reaction diagram
-
-
-
-
?
ATP + L-tryptophan + Archeoglobus fulgidus tRNATrp(A73, wild-type)
AMP + diphosphate + Archeoglobus fulgidus L-tryptophanyl-tRNATrp(A73, wild-type)
show the reaction diagram
preference profile regarding the discriminator base 73 of different tRNA(Trp) substrates in the order of decreasing efficiency is A, C, U, G. Modest preferences for A73 tRNA(Trp)
-
-
?
ATP + L-tryptophan + Archeoglobus fulgidus tRNATrp(A73C)
AMP + diphosphate + Archeoglobus fulgidus L-tryptophanyl-tRNATrp(A73C)
show the reaction diagram
preference profile regarding the discriminator base 73 of different tRNA(Trp) substrates in the order of decreasing efficiency is A, C, U, G. Modest preferences for A73 tRNA(Trp)
-
-
?
ATP + L-tryptophan + Archeoglobus fulgidus tRNATrp(A73G)
AMP + diphosphate + Archeoglobus fulgidus L-tryptophanyl-tRNATrp(A73G)
show the reaction diagram
preference profile regarding the discriminator base 73 of different tRNA(Trp) substrates in the order of decreasing efficiency is A, C, U, G. Modest preferences for A73 tRNA(Trp)
-
-
?
ATP + L-tryptophan + Archeoglobus fulgidus tRNATrp(A73U)
AMP + diphosphate + Archeoglobus fulgidus L-tryptophanyl-tRNATrp(A73U)
show the reaction diagram
preference profile regarding the discriminator base 73 of different tRNA(Trp) substrates in the order of decreasing efficiency is A, C, U, G. Modest preferences for A73 tRNA(Trp)
-
-
?
ATP + L-tryptophan + Bacillus subtilis tRNATrp(A73, wild-type)
AMP + diphosphate + Bacillus subtilis L-tryptophanyl-tRNATrp(A73, wild-type)
show the reaction diagram
preference profile regarding the discriminator base 73 of different tRNA(Trp) substrates in the order of decreasing efficiency is A, C, U, G. Modest preferences for A73 tRNA(Trp)
-
-
?
ATP + L-tryptophan + Bacillus subtilis tRNATrp(A73C)
AMP + diphosphate + Bacillus subtilis L-tryptophanyl-tRNATrp(A73C)
show the reaction diagram
preference profile regarding the discriminator base 73 of different tRNA(Trp) substrates in the order of decreasing efficiency is A, C, U, G. Modest preferences for A73 tRNA(Trp)
-
-
?
ATP + L-tryptophan + Bacillus subtilis tRNATrp(A73G)
AMP + diphosphate + Bacillus subtilis L-tryptophanyl-tRNATrp(A73G)
show the reaction diagram
preference profile regarding the discriminator base 73 of different tRNA(Trp) substrates in the order of decreasing efficiency is A, C, U, G. Modest preferences for A73 tRNA(Trp)
-
-
?
ATP + L-tryptophan + Bacillus subtilis tRNATrp(A73U)
AMP + diphosphate + Bacillus subtilis L-tryptophanyl-tRNATrp(A73U)
show the reaction diagram
preference profile regarding the discriminator base 73 of different tRNA(Trp) substrates in the order of decreasing efficiency is A, C, U, G. Modest preferences for A73 tRNA(Trp)
-
-
?
ATP + L-tryptophan + bovine tRNATrp(G73, wild-type)
AMP + diphosphate + bovine L-tryptophanyl-tRNATrp(G73, wild-type)
show the reaction diagram
preference profile regarding the discriminator base 73 of different tRNA(Trp) substrates in the order of decreasing efficiency is A, C, U, G. Modest preferences for A73 tRNA(Trp)
-
-
?
ATP + L-tryptophan + bovine tRNATrp(G73A)
AMP + diphosphate + bovine L-tryptophanyl-tRNATrp(G73A)
show the reaction diagram
preference profile regarding the discriminator base 73 of different tRNA(Trp) substrates in the order of decreasing efficiency is A, C, U, G. Modest preferences for A73 tRNA(Trp)
-
-
?
ATP + L-tryptophan + bovine tRNATrp(G73C)
AMP + diphosphate + bovine L-tryptophanyl-tRNATrp(G73C)
show the reaction diagram
preference profile regarding the discriminator base 73 of different tRNA(Trp) substrates in the order of decreasing efficiency is A, C, U, G. Modest preferences for A73 tRNA(Trp)
-
-
?
ATP + L-tryptophan + bovine tRNATrp(G73U)
AMP + diphosphate + bovine L-tryptophanyl-tRNATrp(G73U)
show the reaction diagram
preference profile regarding the discriminator base 73 of different tRNA(Trp) substrates in the order of decreasing efficiency is A, C, U, G. Modest preferences for A73 tRNA(Trp)
-
-
?
ATP + L-tryptophan + tRNAArg
AMP + diphosphate + L-tryptophyl-tRNAArg
show the reaction diagram
-
-
-
-
?
ATP + L-tryptophan + tRNATrp
?
show the reaction diagram
ATP + L-tryptophan + tRNATrp
AMP + diphosphate + L-tryptophan-tRNATrp
show the reaction diagram
ATP + L-tryptophan + tRNATrp
AMP + diphosphate + L-tryptophanyl-tRNATrp
show the reaction diagram
ATP + L-tryptophan + tRNATrp
AMP + diphosphate + L-tryptophyl-tRNATrp
show the reaction diagram
ATP + L-tryptophan + tRNATrp(A36C)
AMP + diphosphate + L-tryptophyl-tRNATrp(A36C)
show the reaction diagram
-
substrate mutant
-
-
?
ATP + L-tryptophan + tRNATrp(A36G)
AMP + diphosphate + L-tryptophyl-tRNATrp(A36G)
show the reaction diagram
-
substrate mutant
-
-
?
ATP + L-tryptophan + tRNATrp(A36U)
AMP + diphosphate + L-tryptophyl-tRNATrp(A36U)
show the reaction diagram
-
substrate mutant
-
-
?
ATP + L-tryptophan + tRNATrp(A36U, A73G)
AMP + diphosphate + L-tryptophyl-tRNATrp(A36U, A73G)
show the reaction diagram
-
substrate mutant
-
-
?
ATP + L-tryptophan + tRNATrp(A73C)
AMP + diphosphate + L-tryptophyl-tRNATrp(A73C)
show the reaction diagram
-
substrate mutant
-
-
?
ATP + L-tryptophan + tRNATrp(A73G)
AMP + diphosphate + L-tryptophyl-tRNATrp(A73G)
show the reaction diagram
-
substrate mutant
-
-
?
ATP + L-tryptophan + tRNATrp(A73U)
AMP + diphosphate + L-tryptophyl-tRNATrp(A73U)
show the reaction diagram
-
substrate mutant
-
-
?
ATP + L-tryptophan + tRNATrp(C34A)
AMP + diphosphate + L-tryptophyl-tRNATrp(C34A)
show the reaction diagram
-
substrate mutant
-
-
?
ATP + L-tryptophan + tRNATrp(C34G)
AMP + diphosphate + L-tryptophyl-tRNATrp(C34G)
show the reaction diagram
-
substrate mutant
-
-
?
ATP + L-tryptophan + tRNATrp(C34U)
AMP + diphosphate + L-tryptophyl-tRNATrp(C34U)
show the reaction diagram
-
substrate mutant
-
-
?
ATP + L-tryptophan + tRNATrp(C35A)
AMP + diphosphate + L-tryptophyl-tRNATrp(C35A)
show the reaction diagram
-
substrate mutant
-
-
?
ATP + L-tryptophan + tRNATrp(C35G)
AMP + diphosphate + L-tryptophyl-tRNATrp(C35G)
show the reaction diagram
-
substrate mutant
-
-
?
ATP + L-tryptophan + tRNATrp(C35U)
AMP + diphosphate + L-tryptophyl-tRNATrp(C35U)
show the reaction diagram
-
substrate mutant
-
-
?
ATP + L-tryptophan + tRNATrp(G1A-C72U)
AMP + diphosphate + L-tryptophyl-tRNATrp(G1A-C72U)
show the reaction diagram
-
substrate mutant
-
-
?
ATP + L-tryptophan + tRNATrp(G1C-G72G)
AMP + diphosphate + L-tryptophyl-tRNATrp(G1C-G72G)
show the reaction diagram
-
substrate mutant
-
-
?
ATP + L-tryptophan + tRNATrp(G1U-C72A)
AMP + diphosphate + L-tryptophyl-tRNATrp(G1U-C72A)
show the reaction diagram
-
substrate mutant
-
-
?
ATP + L-tryptophan + tRNATrp(G2A-C71U)
AMP + diphosphate + L-tryptophyl-tRNATrp(G2A-C71U)
show the reaction diagram
-
substrate mutant
-
-
?
ATP + L-tryptophan + tRNATrp(G2C-G71G)
AMP + diphosphate + L-tryptophyl-tRNATrp(G2C-G71G)
show the reaction diagram
-
substrate mutant
-
-
?
ATP + L-tryptophan + tRNATrp(G2U-C71A)
AMP + diphosphate + L-tryptophyl-tRNATrp(G2U-C71A)
show the reaction diagram
-
substrate mutant
-
-
?
ATP + L-tryptophan + tRNATrp(HOG1)
AMP + diphosphate + L-tryptophyl-tRNATrp(HOG1)
show the reaction diagram
-
substrate mutant
-
-
?
ATP + L-tryptophan + tRNATrp(pppG1)
AMP + diphosphate + L-tryptophyl-tRNATrp(pppG1)
show the reaction diagram
-
substrate mutant
-
-
?
L-tryptophan + ATP
L-Trp-adenylate + diphosphate
show the reaction diagram
the enzyme also catalyzes the exchange of diphosphate in the diphosphate-ATP exchange assay
-
r
L-tryptophan + ATP + ADP
P1,P3-bis(5'-adenosyl)triphosphate + ?
show the reaction diagram
-
-
-
-
L-tryptophan + ATP + NH3
L-tryptophanamide + AMP + diphosphate
show the reaction diagram
-
-
-
-
P1,P3-bis(5'-adenosyl)triphosphate + L-tryptophan + tRNATrp
?
show the reaction diagram
-
-
-
-
-
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ATP + L-tryptophan + tRNATrp
?
show the reaction diagram
ATP + L-tryptophan + tRNATrp
AMP + diphosphate + L-tryptophan-tRNATrp
show the reaction diagram
ATP + L-tryptophan + tRNATrp
AMP + diphosphate + L-tryptophanyl-tRNATrp
show the reaction diagram
ATP + L-tryptophan + tRNATrp
AMP + diphosphate + L-tryptophyl-tRNATrp
show the reaction diagram
additional information
?
-
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
KCl
-
optimal concentration: 50 mM
Mn2+
-
replacing Mg2+ with Mn2+ reduces the specificity for L-tryptophan, increasing the relative activation of L-tyrosine by about 3.5fold
additional information
-
no effects by Fe2+ and diphosphate
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1,10-phenanthroline
-
-
2'-O-methyladenosine
-
-
2-[5-[(6-(ethoxycarbonyl)-5-(4-fluorophenyl)-3-oxo-7-phenyl-5H-[1,3]thiazolo[3,2-a]pyrimidin-2(3H)-ylidene)methyl]-2-furyl]benzoic acid
-
3'-O-Methyl-ATP
-
ATP-diphosphate exchange, noncompetitive with respect to ATP
3'-O-Methyladenosine
-
ATP-diphosphate exchange, noncompetitive with respect to ATP
3-[(4-[[4-oxo-2-(phenylimino)-1,3-thiazolidin-5-ylidene]methyl]phenoxy) methyl]benzoic acid
-
3-[[([[2-(4-chlorophenyl)-4-quinolinyl]carbonyl]amino)carbothioyl]amino]benzoic acid
-
4,5,6,7-Tetrafluorotryptophan
-
-
5,7-Difluorotryptophan
-
-
5-hydroxytryptophan
-
ATP-diphosphate exchange, weak
5-Methyltryptophan
-
ATP-diphosphate exchange, weak
6-methyltryptophan
-
ATP-diphosphate exchange, weak
9-(2',3'-Dihydroxypropyl)adenine
-
S-isomer
9-(2'-Hydroxyethyl)adenine
-
-
9-(2'-Hydroxyethyl)adenine triphosphate
-
-
9-(3'-Hydroxypropyl)adenine
-
-
9-(3'-Hydroxypropyl)adenine triphosphate
-
-
9-(4'-Hydroxybutyl)adenine
-
-
9-(4'-Hydroxybutyl)adenine triphosphate
-
-
adenine
-
-
adenosine
-
-
Adenosine-5'-(beta-bromoethane phosphonate)
-
-
Adenosine-5'-(beta-bromoethanediphosphonate)
-
-
Adenosine-5'-(beta-chloroethyl phosphate)
-
-
Adenosine-5'-chloromethylphosphonate
-
ATP-diphosphate exchange, reversible, incompetitive
alpha,beta-methylene-ATP
-
-
beta-Indolyl propionic acid
-
-
beta-indolylacetic acid
-
-
beta-Indolylpyruvic acid
-
-
beta-Methyl-DL-tryptophan
-
ATP-diphosphate exchange
chuangximycin aldehyde
-
strong inhibition
chuangximycin lactone
-
-
chuangximycin nitrile
-
-
chuangxinmycin
D-tryptophan
-
-
dATP
-
-
diethyl dicarbonate
-
substrates protect, completely reactivated by hydroxylamine
DL-Tryptophanyl hydroxamate
-
ATP-diphosphate exchange
gamma-(n-Azidoanilide)-ATP
-
-
indolmycin
L-tryptophan
Mixed anhydride of ADP and mesithylenecarboxylic acid
-
-
-
Mixed anhydride of AMP and mesithylenecarboxylic acid
-
-
-
N-acetyl-L-tryptophan
-
-
N-Formyl-L-tryptophan
-
-
p-hydroxymercuribenzoate
-
-
succinylacetone
-
a heme biosynthesis inhibitor, inhibit cellular TrpRS activity in IFN-gamma-activated cells without affecting TrpRS protein expression
tryptamine
additional information
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
glyceraldehyde 3-phosphate dehydrogenase
-
the oxidized form of glyceraldehyde 3-phosphate dehydrogenase interacts with both full-length and mini tryptophanyl-tRNA synthetase and specifically stimulates the aminoacylation potential of mini, but not full-length tryptophanyl-tRNA synthetase. In contrast, reduced glyceraldehyde 3-phosphate dehydrogenase does not bind to tryptophanyl-tRNA synthetase and does not influence their aminoacylation activity
-
Hemin
-
heme can interact strongly with Zn2+-depleted full-length TrpRS, but not the Zn2+-bound enzyme, with a stoichiometric heme:protein ratio of 1:1 to enhance the aminoacylation activity significantly, involvement of heme in regulation of TrpRS aminoacylation activity, overview
interferon gamma
-
interferon regulatory factor 1
-
stimulates and regulates the enzyme isoforms expression via 2 specific interferon-gamma-sensitive promoters that are additionally regulated by the interferon gamma
-
interferons
-
interferons alpha, beta, and gamma, induction of accumulation of the enzyme, organelle-specific, the highest content is observed in the cytoplasm, followd by the nucleolus and the nucleus, the latter with a low enzyme content
-
additional information
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.00006
Archeoglobus fulgidus tRNATrp(A73, wild-type)
pH 7.0, 65°C
-
0.00005
Archeoglobus fulgidus tRNATrp(A73C)
pH 7.0, 65°C
-
0.00006
Archeoglobus fulgidus tRNATrp(A73G)
pH 7.0, 65°C
-
0.00005
Archeoglobus fulgidus tRNATrp(A73U)
pH 7.0, 65°C
-
0.03 - 2
ATP
0.00009
Bacillus subtilis tRNATrp(A73, wild-type)
pH 7.0, 65°C
-
0.00012
Bacillus subtilis tRNATrp(A73C)
pH 7.0, 65°C
-
0.0001
Bacillus subtilis tRNATrp(A73G)
pH 7.0, 65°C
-
0.00012
Bacillus subtilis tRNATrp(A73U)
pH 7.0, 65°C
-
0.00018
bovine tRNATrp(G73, wild-type)
pH 7.0, 65°C
-
0.00021
bovine tRNATrp(G73A)
pH 7.0, 65°C
-
0.00022
bovine tRNATrp(G73C)
pH 7.0, 65°C
-
0.00017
bovine tRNATrp(G73U)
pH 7.0, 65°C
-
0.00073 - 0.027
L-tryptophan
1
P1,P3-bis(5'-adenosyl)triphosphate
0.0021
tRNAArg
-
pH 7.5, 60°C
0.00012 - 0.0248
tRNATrp
0.0059
tRNATrp(A36C)
-
substrate mutant, pH 7.5, 60°C
-
0.0053
tRNATrp(A36G)
-
substrate mutant, pH 7.5, 60°C
-
0.0058
tRNATrp(A36U)
-
substrate mutant, pH 7.5, 60°C
-
0.0098
tRNATrp(A36U, A73G)
-
substrate mutant, pH 7.5, 60°C
-
0.0017
tRNATrp(A73C)
-
substrate mutant, pH 7.5, 60°C
-
0.0058
tRNATrp(A73G)
-
substrate mutant, pH 7.5, 60°C
-
0.0051
tRNATrp(A73U)
-
substrate mutant, pH 7.5, 60°C
-
0.06
tRNATrp(C34A)
-
substrate mutant, pH 7.5, 60°C
-
0.038
tRNATrp(C34G)
-
substrate mutant, pH 7.5, 60°C
-
0.048
tRNATrp(C34U)
-
substrate mutant, pH 7.5, 60°C
-
0.014
tRNATrp(C35A)
-
substrate mutant, pH 7.5, 60°C
-
0.038
tRNATrp(C35G)
-
substrate mutant, pH 7.5, 60°C
-
0.021
tRNATrp(C35U)
-
substrate mutant, pH 7.5, 60°C
-
0.025
tRNATrp(G1A-C72U)
-
substrate mutant, pH 7.5, 60°C
-
0.0055
tRNATrp(G1C-G72G)
-
substrate mutant, pH 7.5, 60°C
-
0.021
tRNATrp(G1U-C72A)
-
substrate mutant, pH 7.5, 60°C
-
0.015
tRNATrp(G2A-C71U)
-
substrate mutant, pH 7.5, 60°C
-
0.0019
tRNATrp(G2C-G71G)
-
substrate mutant, pH 7.5, 60°C
-
0.0058
tRNATrp(G2U-C71A)
-
substrate mutant, pH 7.5, 60°C
-
0.006
tRNATrp(HOG1)
-
substrate mutant, pH 7.5, 60°C
-
0.012
tRNATrp(pppG1)
-
substrate mutant, pH 7.5, 60°C
-
0.00045 - 0.128
Trp
additional information
additional information
-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
5.7
ammonia
Escherichia coli
-
-
0.53
Archeoglobus fulgidus tRNATrp(A73, wild-type)
Archaeoglobus fulgidus
O28579
pH 7.0, 65°C
-
0.11
Archeoglobus fulgidus tRNATrp(A73C)
Archaeoglobus fulgidus
O28579
pH 7.0, 65°C
-
0.07
Archeoglobus fulgidus tRNATrp(A73G)
Archaeoglobus fulgidus
O28579
pH 7.0, 65°C
-
0.09
Archeoglobus fulgidus tRNATrp(A73U)
Archaeoglobus fulgidus
O28579
pH 7.0, 65°C
-
1.1 - 419
ATP
0.14
Bacillus subtilis tRNATrp(A73, wild-type)
Archaeoglobus fulgidus
O28579
pH 7.0, 65°C
-
0.06
Bacillus subtilis tRNATrp(A73C)
Archaeoglobus fulgidus
O28579
pH 7.0, 65°C
-
0.04
Bacillus subtilis tRNATrp(A73G)
Archaeoglobus fulgidus
O28579
pH 7.0, 65°C
-
0.05
Bacillus subtilis tRNATrp(A73U)
Archaeoglobus fulgidus
O28579
pH 7.0, 65°C
-
0.03
bovine tRNATrp(G73, wild-type)
Archaeoglobus fulgidus
O28579
pH 7.0, 65°C
-
0.08
bovine tRNATrp(G73A)
Archaeoglobus fulgidus
O28579
pH 7.0, 65°C
-
0.06
bovine tRNATrp(G73C)
Archaeoglobus fulgidus
O28579
pH 7.0, 65°C
-
0.04
bovine tRNATrp(G73U)
Archaeoglobus fulgidus
O28579
pH 7.0, 65°C
-
0.59 - 24.5
L-tryptophan
0.16
tRNAArg
Aeropyrum pernix K1
-
pH 7.5, 60°C
0.0009 - 16
tRNATrp
11
tRNATrp(A36C)
Aeropyrum pernix K1
-
substrate mutant, pH 7.5, 60°C
-
4.4
tRNATrp(A36G)
Aeropyrum pernix K1
-
substrate mutant, pH 7.5, 60°C
-
21
tRNATrp(A36U)
Aeropyrum pernix K1
-
substrate mutant, pH 7.5, 60°C
-
0.27
tRNATrp(A36U, A73G)
Aeropyrum pernix K1
-
substrate mutant, pH 7.5, 60°C
-
5.5
tRNATrp(A73C)
Aeropyrum pernix K1
-
substrate mutant, pH 7.5, 60°C
-
0.62
tRNATrp(A73G)
Aeropyrum pernix K1
-
substrate mutant, pH 7.5, 60°C
-
6.1
tRNATrp(A73U)
Aeropyrum pernix K1
-
substrate mutant, pH 7.5, 60°C
-
0.17
tRNATrp(C34A)
Aeropyrum pernix K1
-
substrate mutant, pH 7.5, 60°C
-
0.13
tRNATrp(C34G)
Aeropyrum pernix K1
-
substrate mutant, pH 7.5, 60°C
-
0.11
tRNATrp(C34U)
Aeropyrum pernix K1
-
substrate mutant, pH 7.5, 60°C
-
0.15
tRNATrp(C35A)
Aeropyrum pernix K1
-
substrate mutant, pH 7.5, 60°C
-
0.079
tRNATrp(C35G)
Aeropyrum pernix K1
-
substrate mutant, pH 7.5, 60°C
-
0.47
tRNATrp(C35U)
Aeropyrum pernix K1
-
substrate mutant, pH 7.5, 60°C
-
2.3
tRNATrp(G1A-C72U)
Aeropyrum pernix K1
-
substrate mutant, pH 7.5, 60°C
-
0.34
tRNATrp(G1C-G72G)
Aeropyrum pernix K1
-
substrate mutant, pH 7.5, 60°C
-
0.46
tRNATrp(G1U-C72A)
Aeropyrum pernix K1
-
substrate mutant, pH 7.5, 60°C
-
19
tRNATrp(G2A-C71U)
Aeropyrum pernix K1
-
substrate mutant, pH 7.5, 60°C
-
1.7
tRNATrp(G2C-G71G)
Aeropyrum pernix K1
-
substrate mutant, pH 7.5, 60°C
-
7.2
tRNATrp(G2U-C71A)
Aeropyrum pernix K1
-
substrate mutant, pH 7.5, 60°C
-
3.2
tRNATrp(HOG1)
Aeropyrum pernix K1
-
substrate mutant, pH 7.5, 60°C
-
4.5
tRNATrp(pppG1)
Aeropyrum pernix K1
-
substrate mutant, pH 7.5, 60°C
-
2 - 419
Trp
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
164 - 1090
L-tryptophan
119
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
additional information
-
-
-
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0187 - 0.0228
2-[5-[(6-(ethoxycarbonyl)-5-(4-fluorophenyl)-3-oxo-7-phenyl-5H-[1,3]thiazolo[3,2-a]pyrimidin-2(3H)-ylidene)methyl]-2-furyl]benzoic acid
0.0151 - 0.0183
3-[(4-[[4-oxo-2-(phenylimino)-1,3-thiazolidin-5-ylidene]methyl]phenoxy) methyl]benzoic acid
0.0352 - 0.0422
3-[[([[2-(4-chlorophenyl)-4-quinolinyl]carbonyl]amino)carbothioyl]amino]benzoic acid
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.0018
-
purified enzyme
0.45
-
-
1.45
-
-
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.5 - 7.5
-
in cacodylate buffer
7.8
-
assay at
8.5 - 9.2
-
in Tris buffer
8.5
-
in Hepes buffer
9
exchange assay at
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
60
-
tryptophanylation assay
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
about 0.2% of total soluble protein
Manually annotated by BRENDA team
-
about 0.2% of total soluble protein
Manually annotated by BRENDA team
-
monocyte-derived mature
Manually annotated by BRENDA team
-
about 0.2% of total soluble protein
Manually annotated by BRENDA team
-
umbilical vein
Manually annotated by BRENDA team
-
monocyte
Manually annotated by BRENDA team
-
CD4+ cell, CD8+ cell
Manually annotated by BRENDA team
-
-
Manually annotated by BRENDA team
-
about 0.2% of total soluble protein
Manually annotated by BRENDA team
-
endothelial cell
Manually annotated by BRENDA team
additional information
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
-
enzyme is secreted into the extracellular region of vascular endothelial cells
-
Manually annotated by BRENDA team
-
low content
Manually annotated by BRENDA team
additional information
PDB
SCOP
CATH
ORGANISM
UNIPROT
Aeropyrum pernix (strain ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 / K1)
Aeropyrum pernix (strain ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 / K1)
Bacillus subtilis (strain 168)
Campylobacter jejuni subsp. jejuni serotype O:2 (strain ATCC 700819 / NCTC 11168)
Campylobacter jejuni subsp. jejuni serotype O:2 (strain ATCC 700819 / NCTC 11168)
Cryptosporidium parvum (strain Iowa II)
Deinococcus radiodurans (strain ATCC 13939 / DSM 20539 / JCM 16871 / LMG 4051 / NBRC 15346 / NCIMB 9279 / R1 / VKM B-1422)
Deinococcus radiodurans (strain ATCC 13939 / DSM 20539 / JCM 16871 / LMG 4051 / NBRC 15346 / NCIMB 9279 / R1 / VKM B-1422)
Deinococcus radiodurans (strain ATCC 13939 / DSM 20539 / JCM 16871 / LMG 4051 / NBRC 15346 / NCIMB 9279 / R1 / VKM B-1422)
Deinococcus radiodurans (strain ATCC 13939 / DSM 20539 / JCM 16871 / LMG 4051 / NBRC 15346 / NCIMB 9279 / R1 / VKM B-1422)
Encephalitozoon cuniculi (strain GB-M1)
Giardia intestinalis (strain ATCC 50803 / WB clone C6)
Mycoplasma pneumoniae (strain ATCC 29342 / M129)
Neisseria gonorrhoeae (strain ATCC 700825 / FA 1090)
Neisseria gonorrhoeae (strain ATCC 700825 / FA 1090)
Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Trypanosoma brucei brucei (strain 927/4 GUTat10.1)
Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
35000
x * 35000, recombinant His-tagged enzyme, expressed in insect cells, SDS-PAGE
37000
-
possibly 4 * 37000, SDS-PAGE
49000
-
transcript variant of WARS, determined by SDS-PAGE and Western blot analysis
54000
-
2 * 54000, enzyme form TRS108, SDS-PAGE
60000
-
2 * 60000, SDS-PAGE
70000 - 83000
-
enzyme form TRS82, gel filtration, equilibrium sedimentation, gel electrophoresis
73000
2 * 73000, SDS-PAGE
88000
-
enzyme form TRS88, gel electrophoresis
108000
-
gel filtration, enzyme form TRS108, equilibrium sedimentation
120000
-
gel filtration
140000
gel filtration
200000
-
gel filtration
additional information
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
x * 35000, recombinant His-tagged enzyme, expressed in insect cells, SDS-PAGE
homodimer
tetramer
additional information