EC Number   |
|---|
  4.2.3.89 | expressed in Streptomyces lividans strain TK21 and as N-terminally histidine-tagged enzyme in Escherichia coli BL21(DE3) cells |
| 4.2.3.89 | gene CsSesquiTPS6b, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, the enzyme encoded by CsSesquiTPS6a produces exclusively beta-caryophyllene, while the enzyme encoded by CsSesquiTPS6b and CsSesquiTPS7 render higher amounts of beta-caryophyllene (about 73% or 75% of total, respectively) together with alpha-humulene (about 23% or 25% of total, respectively) |
| 4.2.3.89 | gene CsSesquiTPS6b, DNA and amino acid sequence determination and analysis, the enzyme encoded by CsSesquiTPS6a produces exclusively beta-caryophyllene, while the enzyme encoded by CsSesquiTPS6b and CsSesquiTPS7 render higher amounts of beta-caryophyllene (about 73% or 75% of total, respectively) together with alpha-humulene (about 23% or 25% of total, respectively) |
| 4.2.3.89 | gene CsSesquiTPS6c, DNA and amino acid sequence determination and analysis, the enzyme encoded by CsSesquiTPS6a produces exclusively beta-caryophyllene, while the enzyme encoded by CsSesquiTPS6b and CsSesquiTPS7 render higher amounts of beta-caryophyllene (about 73% or 75% of total, respectively) together with alpha-humulene (about 23% or 25% of total, respectively) |
| 4.2.3.89 | gene DcTPS1, cloned from wild Daucus carrota carota fruits (accession 23727), unrooted neighbor-joining phylogenetic tree, recombinant expression of C-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3) |
| 4.2.3.89 | gene OkBCS, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, quantitative RT-PCR expression analysis, recombinant expression of C-terminally His6-tagged enzyme in Escherichia coli TOP10 cells, transient overexpression in Ocimum kilimandscharicum via Agrobacterium tumefaciens strain GV3101 mediated transfection |
| 4.2.3.89 | gene PlCAHS, DNA and amino acid sequence determination and analysis,sequence comparisons and phylogenetic analysis, real time quantitative PCR expression analysis, recombinant expression in Escherichia coli strain BL21(DE3) |
| 4.2.3.89 | gene TPS1, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis and tree, the enzyme encoded by gene TPS1 produces beta-caryophyllene as a main product and humulene as a minor compound, and thus is named caryophyllene synthase (PnCPS), illumina transcriptome sequencing of unripe peppercorn identifiying sesquiterpene synthases (sesqui-TPSs), recombinant expression of His-tagged enzyme TPS1 in Escherichia coli strain Rosetta 2(DE3) |
| 4.2.3.89 | gene TPS1, sequence comparisons and phylogenetic tree, isozyme TPS1 RT-PCR expression analysis, recombinant expression of His-tagged isozyme in Escherichia coli strain BL21(DE3) |
| 4.2.3.89 | gene tps23, recombinant overexpression in Escherichia coli strain YJM59, coexpression with geranyl diphosphate synthase (GPPS2 gene from Abies grandis), glucose-6-phosphate dehydrogenase (G6PDH gene) from plasmid pACY-mvaE-mvaS-QHS1-GPPS2-G6PDH |
