Information on EC 4.2.3.89 - (+)-beta-caryophyllene synthase

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The expected taxonomic range for this enzyme is: Streptomyces griseus

EC NUMBER
COMMENTARY
4.2.3.89
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RECOMMENDED NAME
GeneOntology No.
(+)-beta-caryophyllene synthase
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
(2E,6E)-farnesyl diphosphate = (+)-beta-caryophyllene + diphosphate
show the reaction diagram
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-
-
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PATHWAY
KEGG Link
MetaCyc Link
Sesquiterpenoid and triterpenoid biosynthesis
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SYSTEMATIC NAME
IUBMB Comments
(2Z,6E)-farnesyl-diphosphate diphosphate-lyase [cyclizing, (+)-beta-caryophyllene-forming]
A multifunctional enzyme which also converts the (+)-beta-caryophyllene to (+)-caryolan-1-ol (see EC 4.2.1.138, (+)-caryolan-1-ol synthase). cf. EC 4.2.3.57 (-)-beta-caryophyllene synthase.
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
GcoA
Streptomyces griseus IFO13350
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-
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ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
Streptomyces griseus IFO13350
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-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(2E,6E)-farnesyl diphosphate + H2O
beta-caryophyllene + diphosphate
show the reaction diagram
Streptomyces griseus, Streptomyces griseus IFO13350
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-
-
-
?
METALS and IONS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
Mg2+
-
more than 5 mM MgCl2 is required for maximum activity. The enzyme reaction is unable to proceed without MgCl2
Mn2+
-
38% activity (relative to the activity in the presence of Mg2+) is retained in the presence of 5 mM Mn2+
additional information
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no activity is observed for Fe2+, Co2+, Zn2+, Ni2+, or Cu2+
KM VALUE [mM]
KM VALUE [mM] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.0000956
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(2E,6E)-farnesyl diphosphate
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at pH 7.5 and 30°C in the presence of 5 mM MgCl2
TURNOVER NUMBER [1/s]
TURNOVER NUMBER MAXIMUM[1/s]
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.025
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(2E,6E)-farnesyl diphosphate
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at pH 7.5 and 30°C in the presence of 5 mM MgCl2
pH OPTIMUM
pH MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
7.5
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-
at 30°C
TEMPERATURE OPTIMUM
TEMPERATURE OPTIMUM MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
30
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-
at pH 7.5
SOURCE TISSUE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
SOURCE
Streptomyces griseus IFO13350
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-
-
Manually annotated by BRENDA team
SUBUNITS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
?
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x * 41000, SDS-PAGE
?
Streptomyces griseus IFO13350
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x * 41000, SDS-PAGE
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Purification/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
Ni-NTA column chromatography
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Cloned/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
expressed in Streptomyces lividans strain TK21 and as N-terminally histidine-tagged enzyme in Escherichia coli BL21(DE3) cells
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