EC Number |
---|
3.6.1.45 | - |
3.6.1.45 | DNA and amino acid sequence determination and analysis, functional overexpression in Escherichia coli |
3.6.1.45 | expressed in Escherichia coli BL21 (DE3) cells |
3.6.1.45 | from the gene ushA |
3.6.1.45 | from the gene ushB |
3.6.1.45 | gene UGPP, overexpression in Escherichia coli strain AD494(DE3), subcloning in strain JM109 |
3.6.1.45 | gene ushA, DNA and amino acid sequence determination and analysis, expression analysis by RT-PCR |
3.6.1.45 | gene ushA, overexpression of the enzyme |
3.6.1.45 | overexpressed in Corynebacterium glutamicum |
3.6.1.45 | overexpression of wild-type and mutant UshA enzyme lacking signal peptide in Escherichia coli 5A-1, UshA lacking the signal peptide is an active cytoplasmic 5' nucleotidase which causes conditional lethality |