Information on EC 3.6.1.45 - UDP-sugar diphosphatase

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The expected taxonomic range for this enzyme is: Bacteria, Eukaryota

EC NUMBER
COMMENTARY
3.6.1.45
-
RECOMMENDED NAME
GeneOntology No.
UDP-sugar diphosphatase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
UDP-sugar + H2O = UMP + alpha-D-aldose 1-phosphate
show the reaction diagram
-
-
-
-
UDP-sugar + H2O = UMP + alpha-D-aldose 1-phosphate
show the reaction diagram
UDP-sugar is the best substrate, although other nucleoside-sugar diphosphates are used as substrates with similar Km values but much lower maximum velocities
-, Q9RQT7
REACTION TYPE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
hydrolysis of diphosphate bonds
-
-
-
-
hydrolysis of phosphoric ester
-, Q9RQT7
-
SYSTEMATIC NAME
IUBMB Comments
UDP-sugar sugarphosphohydrolase
A divalent cation is required for activity. UDP-sugar is the best substrate, although other nucleoside-sugar diphosphates are used as substrates with similar Km values but much lower maximum velocities. Thus, this enzyme has a specificity distinct from that of ADP-sugar diphosphatase (EC 3.6.1.21). Some but not all enzymes of this class also appear to have 5'-nucleotidase (see EC 3.1.3.5) activity.
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
5'-nucleotidase/UDP-sugar hydrolase
-
-
5'-nucleotidase/UDP-sugar hydrolase
A4URQ8
-
5'-nucleotidase/UDP-sugar hydrolase
Yersinia intermedia CECT 7230
A4URQ8
-
-
hydrolase, uridine diphosphoglucose (Escherichia coli precursor reduced)
-
-
-
-
hydrolase, uridine diphosphoglucose (Escherichia coli reduced)
-
-
-
-
hydrolase, uridine diphosphoglucose (Salmonella typhimurium clone pAGS5 gene ushB isoenzyme reduced)
-
-
-
-
hydrolase, uridine diphosphoglucose (Salmonella typhimurium gene ushA0 isoenzyme precursor reduced)
-
-
-
-
hydrolase, uridine diphosphoglucose (Salmonella typhimurium gene ushA0 isoenzyme reduced)
-
-
-
-
nucleosidediphosphate-sugar diphosphatase
-
-
-
-
nucleosidediphosphate-sugar pyrophosphatase
-
-
-
-
Nudix hydrolase-like nucleotide hydrolase
-
-
toxin A
Clostridium difficile
-
-
toxin B
Clostridium difficile
-
-
UDP-glucose pyrophosphatase
-
-
UDP-glucose pyrophosphatase
Q9D142
-
UDP-glucose pyrophosphatase
-
-
UDP-sugar diphosphatase
-
-
-
-
UDP-sugar hydrolase
-
-
-
-
UDP-sugar hydrolase
Q9RQT7
-
UDP-sugar pyrophosphatase
-
-
-
-
UDPG pyrophosphatase
-
-
UGPPase
-
-
UGPPase
Q9D142
-
UGPPase
-
-
UshA
-
secreted enzyme with UDP-sugar hydrolase and 5' nucleotidase activities
UshA
-
bifunctional enzyme with UDP-sugar hydrolase and 5' nucleotidase activity
UshA
A4URQ8
-
UshA
Yersinia intermedia CECT 7230
A4URQ8
-
-
UshB protein
-
-
CAS REGISTRY NUMBER
COMMENTARY
103716-25-2
-, hydrolase, uridine diphosphoglucose (Escherichia coli precursor reduced)
103716-26-3
-, hydrolase, uridine diphosphoglucose (Escherichia coli reduced)
106528-92-1
hydrolase, uridine diphosphoglucose (Salmonella typhimurium gene ushA0 isoenzyme precursor reduced)
106528-93-2
hydrolase, uridine diphosphoglucose (Salmonella typhimurium gene ushA0 isoenzyme reduced)
127497-57-8
hydrolase, uridine diphosphoglucose (Salmonella typhimurium clone pAGS5 gene ushB isoenzyme reduced)
57127-20-5
-
ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
Clostridium difficile
2 different enzyme forms: toxin A and toxin B
-
-
Manually annotated by BRENDA team
strain ATCC 13032, expression is phosphate starvation inducible
-
-
Manually annotated by BRENDA team
strain IFO 12010, gene ushA
UniProt
Manually annotated by BRENDA team
ATCC 12793
-
-
Manually annotated by BRENDA team
encoded by the gene ushA, that is nonhomologous to the gene ushB, that encodes the enzyme from Salmonella typhimurium. Escherichia coli also contains a silent allel of the ushB gene, i.e. ushB0
-
-
Manually annotated by BRENDA team
gene ushA
-
-
Manually annotated by BRENDA team
K12 TG1 and K12 5A-1 strains
-
-
Manually annotated by BRENDA team
gene UGPP
Uniprot
Manually annotated by BRENDA team
encoded by the gene ushB, that is nonhomologous to the gene ushA, that encodes the enzyme from Escherichia coli. Salmonella typhimurium also contains a silent allel of the gene ushA, i.e. ushA0; Salmonella enterica serotype typhimurium
-
-
Manually annotated by BRENDA team
strain CECT 7230, gene ushA
UniProt
Manually annotated by BRENDA team
Yersinia intermedia CECT 7230
strain CECT 7230, gene ushA
UniProt
Manually annotated by BRENDA team
TURNOVER NUMBER [1/s]
TURNOVER NUMBER MAXIMUM[1/s]
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
71
-
UDP-glucose
-
pH 7.5, 37°C
97
-
UDP-glucose
A4URQ8, -
pH 7.5, 37°C
SPECIFIC ACTIVITY [µmol/min/mg]
SPECIFIC ACTIVITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
0.076
-
-
endogenous UDP-sugar hydrolase activity 24 hours after the onset of phosphate starvation
0.55
-
-
UDP-sugar hydrolase activity of overexpressed UshA enzyme
12.58
-
-
-
additional information
-
-
endogenous UDP-sugar hydrolase activity not detectable before the shift to phosphate-free medium; UDP-sugar hydrolase activity detectable in cell extracts of phosphate-starved wild-type delta-ushA cells
TEMPERATURE OPTIMUM
TEMPERATURE OPTIMUM MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
37
40
Clostridium difficile
-
-
37
-
-
assay at
37
-
A4URQ8, -
assay at
TEMPERATURE STABILITY
TEMPERATURE STABILITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
additional information
-
-
Co2+, Mn2+ or Mg2+ protect against heat inactivation