EC Number   |
|---|
    3.2.1.1 | - |
| 3.2.1.1 | acid-stable amylase |
| 3.2.1.1 | AMY1, DNA and amino acid sequence determination and analysis, cloning in Escherichia coli strain DH5alpha, expression of wild-type and mutant enzymes in Pichia pastoris strain GS115 |
| 3.2.1.1 | AMY1, subcloning in Escherichia coli strain DH5alpha, expression of the alpha-amylase C-terminally fused to the Aspergillus niger glucoamlyase starch binding domain in Aspergillus niger strain AB4.1, the mutant enzyme is secreted to the culture medium due to the signal peptide of the barley alpha-amylase |
| 3.2.1.1 | AmyD |
| 3.2.1.1 | cloned and overexpressed in Escherichia coli K12TB1 |
| 3.2.1.1 | cloned and overexpressed in Pichia pastoris, recombinant isoenzymes with a six-histidine fusion tag |
| 3.2.1.1 | cloned in Escherichia coli DH5alpha and XL10-Gold to pPICZA-AMY1. pPICZA-AMY1 and the mutants Y105A/Y380A, Y105A/Y380M, H395A, Y380A/H395A, Y380A, Y380M, and Y105A are expressed in Pichia pastoris |
| 3.2.1.1 | cloning in Escherichia coli and Streptomyces sp. |
| 3.2.1.1 | cloning in Escherichia coli strain XL1-Blue and recombinant expression of His-tagged wild-type and mutant His-tagged enzymes in Escherichia coli strain BL21(DE3) |
