EC Number   |
|---|
   2.7.11.25 | - |
| 2.7.11.25 | amplified MEKK3 PB1 DNA fragments inserted between NdeI/XhoI sites of pET22b and wild-type MEKK3 PB1 with a C-terminal His tag expressed in Escherichia coli BL21 (DE3) cells, MEKK3 PB1 mutants expressed in Escherichia coli Rosetta cells |
| 2.7.11.25 | B-RAf, DNA and amino acid sequence determination and analysis, expression of His6-tagged B-Raf in bacteria, expression of His6-tagged and FLAG-tagged C-Raf in insect cells |
| 2.7.11.25 | BcOS4 gene, DNA and amino acid sequence determination and analysis |
| 2.7.11.25 | bioinformatics analysis of the entire maize genome identifies 74 MAPKKK genes, sequence comparisons and phylogenetic analysis and tree, genomic structure, and chromosomal distributions and exon-intron structures of maize MAPKKK genes, real-time quantitative RT-PCR expression analysis of the MAPKKKs. ZmMAPKKK ORF lengths range from 1062 bp (ZmMAPKKK57) to 4014 bp (ZmMAPKKK14) |
| 2.7.11.25 | co-expression of FLAG-tagged MTK1 and wild-type and mutant HA-tagged MKK6 in COS-7 cells, direct and immunoprecipitation study |
| 2.7.11.25 | DNA and amino acid sequence determination and analysis, overexpression of the enzyme can complement the defective mutant and results in increase crippled growth after infection |
| 2.7.11.25 | DNA and amino acid sequence determination and analysis, sequence comparisons, overview |
| 2.7.11.25 | DNA and amino acid sequence determination and analysis, sequence comparisons, overview, MAPKKKalpha overexpression in leaves using the Agrobacterium tumefaciens transfection system |
| 2.7.11.25 | expressed as native and mutant FLAG-tag/HA-tag fusion protein in HEK293T cells, coexpression expreiments with TAK1 |
