EC Number   |
|---|
    2.5.1.29 | - |
| 2.5.1.29 | a short and a long clone, which differ in the substrate specificity, DNA and amino acid sequence determination and analysis, genetic organization, functional complementation of an Escherichia coli strain carrying an Erwinia uredovora gene cluster encoding all pathway enzymes needed for carotenoid biosynthesis, except for GGPPS, expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) |
| 2.5.1.29 | astaxanthin yield in Saccharomyces cerevisiae is enhanced by combinational metabolic engineering and protein engineering targeting at the insufficient precursor supply and the weak downstream pathway capacity. Through introducing the positive GGPP synthase mutant CrtE03M together with overexpression of tHMG1, CrtI and CrtYB (from the cDNA of Xanthophyllomyces dendrorhous), the supply of the precursor beta-carotene is increased. Efficient conversion of beta-carotene to astaxanthin is achieved through increasing beta-carotene ketolase activity via directed evolution |
| 2.5.1.29 | DNA and amino acid sequence determination and analysis |
| 2.5.1.29 | DNA and amino acid sequence determination and analysis, expression in Escherichia coli strain BL21(DE3), functional complementation of the carotenogenic crt gene cluster in Escherichia coli, YFP-tagged GGPPS expression in Catharanthus roseus cell culture |
| 2.5.1.29 | DNA and amino acid sequence determination and analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain Rosetta (DE3) |
| 2.5.1.29 | expression in BL21 gold bacteria |
| 2.5.1.29 | expression in Escherichia coli |
| 2.5.1.29 | expression in Escherichia coli and in Picea abies embryogenic tissue |
| 2.5.1.29 | expression in Escherichia coli as His-tagged protein |
