EC Number |
Substrates |
Organism |
Products |
Reversibility |
---|
2.1.1.219 | 2 S-adenosyl-L-methionine + adenine57/adenine58 in tRNA |
- |
Pyrococcus abyssi |
2 S-adenosyl-L-homocysteine + N1-methyladenine57/N1-methyladenine58 in tRNA |
- |
? |
2.1.1.219 | 2 S-adenosyl-L-methionine + adenine57/adenine58 in tRNA |
the solvent accessibility of the S-adenosyl-L-methionine pocket is not affected by the tRNA, thereby enabling S-adenosyl-L-homocysteine to be replaced by S-adenosyl-L-methionine without prior release of monomethylated tRNA |
Pyrococcus abyssi |
2 S-adenosyl-L-homocysteine + N1-methyladenine57/N1-methyladenine58 in tRNA |
- |
? |
2.1.1.219 | 2 S-adenosyl-L-methionine + adenine57/adenine58 in tRNA |
the solvent accessibility of the S-adenosyl-L-methionine pocket is not affected by the tRNA, thereby enabling S-adenosyl-L-homocysteine to be replaced by S-adenosyl-L-methionine without prior release of monomethylated tRNA |
Pyrococcus abyssi GE5 / CNCM I-1302 / DSM 25543 |
2 S-adenosyl-L-homocysteine + N1-methyladenine57/N1-methyladenine58 in tRNA |
- |
? |
2.1.1.219 | 2 S-adenosyl-L-methionine + adenine57/adenine58 in tRNA |
- |
Pyrococcus abyssi GE5 / CNCM I-1302 / DSM 25543 |
2 S-adenosyl-L-homocysteine + N1-methyladenine57/N1-methyladenine58 in tRNA |
- |
? |
2.1.1.219 | 2 S-adenosyl-L-methionine + adenine57/adenine58 in tRNAArg |
modified mini-tRNAAsp substrate, PabTrmI recognizes and methylates only A58 in mini-tRNAArgTCT containing the G57A58A59U60 sequence |
Pyrococcus abyssi |
2 S-adenosyl-L-homocysteine + adenine57/N1-methyladenine58 in tRNAArg |
- |
? |
2.1.1.219 | 2 S-adenosyl-L-methionine + adenine57/adenine58 in tRNAAsp |
- |
Pyrococcus abyssi |
2 S-adenosyl-L-homocysteine + N1-methyladenine57/N1-methyladenine58 in tRNAAsp |
- |
? |
2.1.1.219 | 2 S-adenosyl-L-methionine + adenine57/adenine58 in tRNAAsp |
the presence of adenine at position 59 in Pyrococcus abyssi tRNA(Asp) is important for the multi-site specificity of the archaeal enzyme at both positions 57 and 58 in tRNAAsp. His78 near the active site is important for efficient catalysis |
Pyrococcus abyssi |
2 S-adenosyl-L-homocysteine + N1-methyladenine57/N1-methyladenine58 in tRNAAsp |
- |
? |
2.1.1.219 | 2 S-adenosyl-L-methionine + adenine57/adenine58 in tRNAAsp |
modified mini-tRNAAsp substrate, PabTrmI recognizes and methylates both A57 and A58 in mini-tRNAAsp containing the A57A58A59U60 sequence |
Pyrococcus abyssi |
2 S-adenosyl-L-homocysteine + N1-methyladenine57/N1-methyladenine58 in tRNAAsp |
- |
? |
2.1.1.219 | 2 S-adenosyl-L-methionine + adenine57/adenine58 in tRNAAsp |
modified mini-tRNAAsp substrate, PabTrmI recognizes and methylates both A57 and A58 in mini-tRNAAsp containing the A57A58A59U60 sequence |
Pyrococcus abyssi GE5 / CNCM I-1302 / DSM 25543 |
2 S-adenosyl-L-homocysteine + N1-methyladenine57/N1-methyladenine58 in tRNAAsp |
- |
? |
2.1.1.219 | 2 S-adenosyl-L-methionine + adenine57/adenine58 in tRNAAsp |
- |
Pyrococcus abyssi GE5 / CNCM I-1302 / DSM 25543 |
2 S-adenosyl-L-homocysteine + N1-methyladenine57/N1-methyladenine58 in tRNAAsp |
- |
? |