EC Number |
Storage Stability |
---|
3.4.11.1 | -15°C |
3.4.11.1 | -20°C, apoenzyme or active enzyme, several years |
3.4.11.1 | -70°C, 100 mM Tris-HCl buffer, pH 8.0, 6 weeks |
3.4.11.1 | -80°C, 10% sucrose, 6 months, more than 90% of the enzyme activity remain |
3.4.11.1 | -80°C, pure enzyme, 0.2 mM MnCl2, 2 weeks, stable |
3.4.11.1 | -80°C, purified enzyme after chloroform/octanol extraction purification step, in presence of 0.2 mM Mn2+, stable for at least 2 weeks |
3.4.11.1 | -80°C, purified enzyme, in 10 mM sodium phosphate, pH 7.8, 0.02 M magnesium acetate, 2 mM DTT, and 0.05 mM manganese chloride, stable for at least 2 months |
3.4.11.1 | 0°C, or -20°C, pH 8.0, 1 year, 70% activity |
3.4.11.1 | 4°C, 0.1 M Tris-HCl buffer, 1 year |
3.4.11.1 | 4°C, partially purified enzyme after ion exchange chromatography purification step, stable for at least 6 months |