EC Number   |
Storage Stability |
|---|
   2.7.3.2 | -10°C, 5 mM Tris/HCl buffer, pH 8.7, 1 mM 2-mercaptoethanol, saturated ammonium sulfate solution |
| 2.7.3.2 | -17°C, inactivation in presence of chloride or nitrate |
| 2.7.3.2 | -70°C, 0.05 M Tris/barbital buffer, pH 7.8, 0.01 M 2-mercaptoethanol, 17% loss of activity in 6 weeks |
| 2.7.3.2 | -80°C, TAP-purified recombinant isoform CK-MB in 50% (v/v) glycerol, 64 weeks, no loss of activity |
| 2.7.3.2 | -80°C, all the samples with 500, 250, and 20 U/l enzyme activity or with 300, 100, and 20 ng/ml mass concentration in 50% (v/v) glycerol, 64 weeks, no loss of activity |
| 2.7.3.2 | 4°C, 10 mM MOPS buffer, pH 7.2, 2% v/v glycerol, 25 mM 2-mercaptoethanol, 0.1 mM EDTA, stable for more than 4 months |
| 2.7.3.2 | 4°C, 20 mM sodium phosphate buffer, pH 8.0, 10% loss of activity in 2 weeks, faster inactivation in presence of dithiothreitol |
| 2.7.3.2 | 4°C, octameric enzyme, protein concentration above 1 mg/ml, 1 mM 2-mercaptoethanol, 0.2 mM EDTA, 0.26 M NaCl, 25 mM sodium phosphate buffer, pH 7.0 |
| 2.7.3.2 | 4°C, pH 7.0, 1 mM dithiothreitol or 14 mM 2-mercaptoethanol, at least 3 months |
| 2.7.3.2 | 4°C, several weeks |
