EC Number |
Specific Activity Minimum [µmol/min/mg] |
Specific Activity Maximum [µmol/min/mg] |
Reference |
---|
3.4.21.1 | -999 |
- |
- |
171865, 171873 |
3.4.21.1 | -999 |
- |
Kh1: 0.25 units with milk-acetate mixture substrate before purification and 1.3 units with milk-acetate mixture substrate after 5.2fold purification at 35°C, Kh2: 0.25 units with milk-acetate mixture substrate before purification and 1.8 units with milk-acetate mixture substrate after 7.2fold purification at 35°C, Kh3: 0.25 units with milk-acetate mixture substrate before purification and 4.1 units with milk-acetate mixture substrate after 16.4fold purification at 35°C (1 unit is defined as the amount of enzyme that clots 5 ml of milk-acetate mixture during 1 min) |
667239 |
3.4.21.1 | -999 |
- |
no hydrolysis of N-benzoyl-L-tyrosine p-nitroanilide |
668324 |
3.4.21.1 | -999 |
- |
the activity towards the most reactive (S)-enantiomer in octane increased 440-fold, increasing the molar ratio of PEG-to-enzyme from 1.1 to 7.1 results in a more than 2fold decrease in enzyme activity |
668042 |
3.4.21.1 | 0.0034 |
- |
casein proteolysis |
650666 |
3.4.21.1 | 0.3221 |
- |
N-succinyl-L-alanyl-L-alanyl-L-prolinyl-L-phenylalanine p-nitroanilide |
668324 |
3.4.21.1 | 0.62 |
- |
crude extract |
708555 |
3.4.21.1 | 1.4 |
- |
substrate benzyloxycarbonyl-L-Ala-L-Ala-L-Leu-4-nitroanilide, pH 8.5, 25°C |
731841 |
3.4.21.1 | 1.59 |
- |
substrate: succinyl-Ala-Ala-Pro-Phe-4-nitroanilide, pH 7.5, 25°C |
753641 |
3.4.21.1 | 4.1 |
- |
after ammonium sulfate fractionation, 6.6fold purified enzyme |
708555 |