Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | during purification, both chymotrypsin A and B are fully activated when extracted | Carassius auratus |
Application | Comment | Organism |
---|---|---|
additional information | potential application value of the two chymotrypsins A and B where low processing temperature and higher enzymatic activity is needed | Carassius auratus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
benzamidine | partially inhibits both chymotrypsin A and B. At room temperature, at 5 mM concentration, 60.3% and 62.5% residual activity for chymotrypsin A and B, respectively | Carassius auratus | |
Cd2+ | partially inactivates both chymotrypsin A and B at concentrations of 1 and 5 mM. At room temperature, at 5 mM concentration, 76.8% and 49.7% residual activity for chymotrypsin A and B, respectively | Carassius auratus | |
chymostatin | effectively inhibits both chymotrypsin A and B. At room temperature, at 0.01 mM concentration, no residual activity for both chymotrypsin A and B | Carassius auratus | |
Cu2+ | strongly inactivates both chymotrypsin A and B at concentrations of 1 and 5 mM. At room temperature, at 5 mM concentration, 0% and 2.5% residual activity for chymotrypsin A and B, respectively | Carassius auratus | |
EDTA | shows minimal inhibitory effect on both chymotrypsin A and B. At room temperature, at 1 mM concentration, 99% and 97% residual activity for chymotrypsin A and B, respectively | Carassius auratus | |
Fe2+ | strongly inactivates both chymotrypsin A and B at concentrations of 1 and 5 mM. At room temperature, at 5 mM concentration, 14.6% and 21.1% residual activity for chymotrypsin A and B, respectively | Carassius auratus | |
Mn2+ | partially inactivates both chymotrypsin A and B at concentrations of 1 and 5 mM. At room temperature, at 5 mM concentration, 85.3% and 48.9% residual activity for chymotrypsin A and B, respectively | Carassius auratus | |
N-tosyl-L-phenylalanine chloromethyl ketone | significant inhibition, especially to chymotrypsin B. At room temperature, at 0.05 mM concentration, 35.6% and 17.5% residual activity for chymotrypsin A and B, respectively | Carassius auratus | |
Pefabloc SC | effectively inhibits both chymotrypsin A and B. At room temperature, at 1 mM concentration, 1.2% and 1.1% residual activity for chymotrypsin A and B, respectively | Carassius auratus | |
pepstatin | shows minimal inhibitory effect on both chymotrypsin A and B. At room temperature, at 0.03 mM concentration, 95.9% and 92.3% residual activity for chymotrypsin A and B, respectively | Carassius auratus | |
phenylmethanesulfonyl fluoride | effectively inhibits both chymotrypsin A and B. At room temperature, at 1 mM concentration, 0.4% and 0.3% residual activity for chymotrypsin A and B, respectively | Carassius auratus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0005 | - |
succinyl-Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin | chymotrypsin B, at pH 7.5 and 37°C | Carassius auratus | |
0.0014 | - |
succinyl-Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin | chymotrypsin A, at pH 7.5 and 37°C | Carassius auratus | |
0.8 | - |
succinyl-Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin | at pH 7.5 and 37°C | Bos taurus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | both chymotrypsin A and B are slightly activated at concentrations of 1 and 5 mM. At room temperature, at 5 mM concentration, 115% and 103% relative activity for chymotrypsin A and B, respectively | Carassius auratus | |
Mg2+ | both chymotrypsin A and B are slightly activated at concentrations of 1 and 5 mM. At room temperature, at 5 mM concentration, 111% and 110% relative activity for chymotrypsin A and B, respectively | Carassius auratus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
27000 | - |
x * 28000, chymotrypsin A, SDS-PAGE. X * 27000, chymotrypsin B, SDS-PAGE | Carassius auratus |
28000 | - |
x * 28000, chymotrypsin A, SDS-PAGE. X * 27000, chymotrypsin B, SDS-PAGE | Carassius auratus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Carassius auratus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
chymotrypsin A and B, to homogeneity by ammonium sulfate fractionation and gel filtration | Carassius auratus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
hepatopancreas | - |
Carassius auratus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.62 | - |
crude extract | Carassius auratus |
4.1 | - |
after ammonium sulfate fractionation, 6.6fold purified enzyme | Carassius auratus |
581.6 | - |
chymotrypsin B, after gel filtration, 938.1fold purified enzyme | Carassius auratus |
981.8 | - |
chymotrypsin A, after gel filtration, 1583fold purified enzyme | Carassius auratus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Gelatin + H2O | chymotrypsin A is active in degrading gelatin, while chymotrypsin B does not reveal activity against gelatin | Carassius auratus | ? | - |
? | |
additional information | chymotrypsin does not reveal activity against gelatin | Bos taurus | ? | - |
? | |
succinyl-Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin + H2O | - |
Bos taurus | succinyl-Leu-Leu-Val-Tyr + 7-amino-4-methylcoumarin | - |
? | |
succinyl-Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin + H2O | chymotrypsin A shows much lower catalytic efficiency to the substrate than chymotrypsin B | Carassius auratus | succinyl-Leu-Leu-Val-Tyr + 7-amino-4-methylcoumarin | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 28000, chymotrypsin A, SDS-PAGE. X * 27000, chymotrypsin B, SDS-PAGE | Carassius auratus |
Synonyms | Comment | Organism |
---|---|---|
chymotrypsin | - |
Bos taurus |
chymotrypsin A | anionic chymotrypsin | Carassius auratus |
chymotrypsin B | cationic chymotrypsin | Carassius auratus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
optimum temperatures of chymotrypsin A and B are 40 and 50°C, respectively, at pH 7.5 using 0.1 M Tris-HCl buffer | Carassius auratus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 45 | chymotrypsin A at 20°C exhibits more than 60% of its maximum activity, which is much higher than chymotrypsin B (27%). Slight changes in activity for both enzymes at 37°C. Relative activities of the two enzymes decrease sharply at above 45°C | Carassius auratus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.9 | - |
succinyl-Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin | at pH 7.5 and 37°C | Bos taurus | |
2.7 | - |
succinyl-Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin | chymotrypsin A, at pH 7.5 and 37°C | Carassius auratus | |
3.4 | - |
succinyl-Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin | chymotrypsin B, at pH 7.5 and 37°C | Carassius auratus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
optimal pH of chymotrypsin A and B are 7.5 and 8.0, respectively, at 37°C | Carassius auratus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 11 | chymotrypsin A and B. The two chymotrypsins are unstable at pH below 5.0, about 10% activity for the enzymes at pH 4.0 | Carassius auratus |
General Information | Comment | Organism |
---|---|---|
physiological function | anti-chymotrypsin B weakly cross reacts with chymotrypsin A | Carassius auratus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1100 | - |
succinyl-Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin | at pH 7.5 and 37°C | Bos taurus | |
1900 | - |
succinyl-Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin | chymotrypsin A, at pH 7.5 and 37°C | Carassius auratus | |
6800 | - |
succinyl-Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin | chymotrypsin B, at pH 7.5 and 37°C | Carassius auratus |