EC Number   |
Reference |
|---|
   2.8.2.20 | functional refolding of recombinant GST-tagged TPST-1 expressed in Escherichia coli strain BL21(DE3) in inclusion bodies using 6 M guanidine hydrochloride (GndHCl), glutathione, and n-dodecyl-beta-D-maltoside |
761815 |
| 2.8.2.20 | recombinant His6-tagged TPST1 (residues Lys43-Leu360) lacking the transmembrane domains from Escherichia coli strain BL21(DE3)pLysS inclusion bodies by ultrafiltration, dilution in 100 mM Na-acetate, pH 4.5, 6 M guanidine hydrochloride, and 10 mM DTT, concentrated TPST (about 150 mg) is slowly added to refolding buffer containing 50 mM Tris-HCl, pH 8.5, 500 mM guanidine hydrochloride, 10 mM NaCl, 0.4 mM KCl, 0.1 mM EDTA, 0.14 mM n-dodecyl beta-D-maltoside, 5 mM reduced glutathione, and 2.5 mM oxidised glutathione. The refolding mixture is then incubated for 20 h at 4°C without mixing and precipitated protein is subsequently removed by centrifugation |
760527 |
| 2.8.2.20 | recombinant His6-tagged TPST2 (residues Gly43-Leu359) lacking the transmembrane domains from Escherichia coli strain BL21(DE3)pLysS inclusion bodies by ultrafiltration, dilution in 100 mM Na-acetate, pH 4.5, 6 M guanidine hydrochloride, and 10 mM DTT, concentrated TPST (about 150 mg) is slowly added to refolding buffer containing 50 mM Tris-HCl, pH 8.5, 500 mM guanidine hydrochloride, 10 mM NaCl, 0.4 mM KCl, 0.1 mM EDTA, 0.14 mM n-dodecyl beta-D-maltoside, 5 mM reduced glutathione, and 2.5 mM oxidised glutathione. The refolding mixture is then incubated for 20 h at 4°C without mixing and precipitated protein is subsequently removed by centrifugation |
760527 |
