EC Number   |
|---|
    6.1.1.20 | - |
| 6.1.1.20 | 60fold |
| 6.1.1.20 | a TALON metal affinity resin column is applied |
| 6.1.1.20 | ammonium sulfate fractionation is followed by four chromatography columns, anion exchange on a DEAE-Sepharose column, a heparin-Sepharose column, concentrating the protein on a TSK hydrophobic interaction column and finally gel filtration on a column of superfine Sephadex G-200 |
| 6.1.1.20 | glutathione Sepharose column chromatography |
| 6.1.1.20 | His6-tagged proteins are purified on nickel-nitrilotriacetic acid-agarose |
| 6.1.1.20 | HiTrap Ni2+ chelating column chromatography, Ni-NTA column chromatography and Sephacryl S300 gel filtration |
| 6.1.1.20 | mature mitPheRS in Escherichia coli by streptomycin precipitation, ultrafiltration, anion exchange chromatography and heparin affinity chromatography, followed by ammonium sulfate fractionation, hydrophobic interaction chromatography, and gel filtration, to homogeneity |
| 6.1.1.20 | native enzyme from Oxford strain, 50% purity, recombinant enzyme overexpressed in Escherichia coli, over 90% purity |
| 6.1.1.20 | Ni-NTA column chromatography |
