EC Number   |
|---|
    3.1.1.4 | - |
| 3.1.1.4 | 3 isoforms: MiPLA-2, MiPLA-3 and MiPLA-4 |
| 3.1.1.4 | 30000fold from rat brain, 90% pure protein |
| 3.1.1.4 | 4 isoforms: SIIISPIIA, SIIISPIIB, SIIISPIIIA, SIIISPIIIB |
| 3.1.1.4 | 560fold |
| 3.1.1.4 | all steps are performed at 4°C, precipitation, gel filtration |
| 3.1.1.4 | ammonium sulfate fractionation, column chromatography |
| 3.1.1.4 | ammonium sulfate precipitation, ethanol fractionation, Mono-S Sepharose column chromatography, Sephadex G-50 gel filtration, RP-HPLC C-8 column chromatography, and Mono-Q column chromatography |
| 3.1.1.4 | by ammonium sulfate and ethanol precipitation and gel filtration, to homogeneity, 250fold. Overall recovery of phospholipase activity is about 82% |
| 3.1.1.4 | by ammonium sulfate and ethanol precipitation, followed by gel filtration and gel filtration, to homogeneity, with a yield of 16%, 2000fold |
