EC Number   |
|---|
   2.7.11.25 | by centrifugation and on Ni2+-NTA resin |
| 2.7.11.25 | by gel filtration |
| 2.7.11.25 | glutathione-Sepharose column chromatography |
| 2.7.11.25 | HisTrap Ni column chromatography |
| 2.7.11.25 | partial purification of Mos and B-Raf from egg extracts by heparin affinity chromatography and/or ion exchange chromatography, and GST-MEK1 affinity chromatography, recombinant His6-tagged B-Raf by nickel affinity chromatography |
| 2.7.11.25 | purification of recombinant MEKK1 and ASK1 by chitin affinity chromatography, endogenous MEKK1 by protein A affinity chromatography |
| 2.7.11.25 | purification of recombinant wild-type and mutant COT30-397s, free or complexed to p105DELTAN, and of p105DELTAN-complexed recombinant COT30-467 from Sf9 insect cells by anti-Flag immunoaffinity chromatography, the purification of free COT30-467 is not possible due to its extremely low expression level in absence of p105DELTAN |
| 2.7.11.25 | recombinant His6- and GST-tagged wild-type and mutants enzymes from Escherichia coli by affinity chromatography and gel filtration, the tags are cleaved off |
| 2.7.11.25 | recombinant protein by tandem affinity purification |
