EC Number   |
|---|
    1.13.11.52 | - |
| 1.13.11.52 | 0-5°C, homogenization, Protein G-Sepharose |
| 1.13.11.52 | ammonium sulfate precipitation, hydroxyapatite column chromatography (two times), gel filtration, DEAE cellulose column chromatography, third hydroxyapatite column chromatography, Sephadex G-200 gel filtration, second DEAE cellulose column chromatography |
| 1.13.11.52 | cryosections deparaffinized, rehydrated, cooked in 0.01 M citrate buffer (pH 6.0), incubated with IDO antibody at room temperature for 30 min |
| 1.13.11.52 | dialysis, lyophilization |
| 1.13.11.52 | enzyme from intestine |
| 1.13.11.52 | expression of a fully functional enzyme in Escherichia coli |
| 1.13.11.52 | HisTrap column chromatography and Superdex 200 gel filtration |
| 1.13.11.52 | homogenization |
| 1.13.11.52 | homogenization, ammonium sulfate precipitation |
