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4.2.1.146
synthesis
extracellular production of L-galactonate in gram quantities from D-galacturonic and polygalacturonic acids. DELTAgaaB strains produce L-galactonate at yields of 0.6 to 0.9 g per g of substrate consumed. Intracellular accumulation of L-galactonate suggests that export may be limiting. Deletion of the L-galactonate dehydratase from Aspergillus niger delays induction of D-galacturonate reductase and overexpression of the reductase improves initial production rates. Deletion of the L-galactonate dehydratase from Aspergillus niger also delays or prevents induction of the putative D-galacturonate transporter An14g04280. Aspergillus nigergaaB produces L-galactonate from polygalacturonate as efficiently as from the monomer
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