Information on EC 4.2.1.146 - L-galactonate dehydratase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
4.2.1.146
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RECOMMENDED NAME
GeneOntology No.
L-galactonate dehydratase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
L-galactonate = 2-dehydro-3-deoxy-L-galactonate + H2O
show the reaction diagram
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
D-galacturonate degradation III
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Metabolic pathways
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Pentose and glucuronate interconversions
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degradation of sugar acids
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SYSTEMATIC NAME
IUBMB Comments
L-galactonate hydro-lyase (2-dehydro-3-deoxy-L-galactonate-forming)
The enzyme takes part in a D-galacturonate degradation pathway in the fungi Trichoderma reesei (Hypocrea jecorina) and Aspergillus niger.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
gene name
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Manually annotated by BRENDA team
gene Bclgd1
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Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
metabolism
additional information
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
D-arabonate
?
show the reaction diagram
50% of the activity with L-galactonate
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?
L-galactonate
2-dehydro-3-deoxy-L-galactonate + H2O
show the reaction diagram
L-galactonate
2-keto-3-deoxy-L-galactonate + H2O
show the reaction diagram
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
L-galactonate
2-dehydro-3-deoxy-L-galactonate + H2O
show the reaction diagram
Q0QWS4
the second enzyme of the fungal pathway for D-galacturonic acid catabolism after the D-galacturonic acid reductase. A deletion of the L-galactonate dehydratase gene in Hypocrea jecorina results in a strain with no growth on D-galacturonic acid
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?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
EDTA
5 mM, complete loss of activity
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
5
L-galactonate
pH 7.0
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.2
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assay at
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
22
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assay at room temperature
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
L-galactonate dehydratase activity is present in cells grown on D-galacturonic acid but absent when other carbon sources are used for growth
Manually annotated by BRENDA team
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
gene Bclgd1, quantitative RT-PCR expression analysis
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the LGD1 protein is tagged with a histidine tag and produced in Saccharomyces cerevisiae. In one construct the C-terminus of the protein was tagged, in another the N-terminal end of the protein
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
glucose induces the enzyme
pectate, pectinate, and galacturonic acid suppress enmzyme expression
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
synthesis