EC Number   |
Application |
Reference |
|---|
   3.5.1.52 | analysis |
a procedure to map N-glycosylation sites is presented, it can be applied to purified proteins as well as to highly complex mixtures. The method exploits deglycosylation by PNGase F in a diagonal, reverse-phase chromatographic setup |
688656 |
| 3.5.1.52 | analysis |
the facile expression, non-glycosylated nature, unusual pH optimum and broad substrate specificity of the enzyme makes recombinant PNGase H+ a versatile tool in N-glycan analysis |
-, 733540 |
| 3.5.1.52 | analysis |
valuable tool to characterize the peptide portion of glycoproteins and glycopeptides to release asparagine-linked oligosaccarides for structural analysis, recombinant PNGase F contains no contaminant Endo F, proteases or exoglycosidases |
288921 |
| 3.5.1.52 | synthesis |
PNGase F can be utilized for glycosylation of non-glycosylated recombinant proteins produced in prokaryotic cells |
668438 |
