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Literature summary for 3.5.1.52 extracted from

  • Barsomian, G.D.; Johnson, T.L.; Borowski, M.; Denman, J.; Ollington, J.F.; Hirani, S.; McNeilly, D.S.; Rasmussen, J.R.
    Cloning and expression of peptide-N4-(N-acetyl-beta-D-glucosaminyl)asparagine amidase F in Escherichia coli (1990), J. Biol. Chem., 265, 6967-6872.
    View publication on PubMed
Search for more literature for 3.5.1.52

Application

Application Comment Organism
analysis valuable tool to characterize the peptide portion of glycoproteins and glycopeptides to release asparagine-linked oligosaccarides for structural analysis, recombinant PNGase F contains no contaminant Endo F, proteases or exoglycosidases Elizabethkingia meningoseptica

Cloned(Commentary)

Cloned (Comment) Organism
-
 Elizabethkingia meningoseptica

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
34700
-
 1 * 34700, native enzyme, 314 amino acid open reading frame Elizabethkingia meningoseptica

Organism

Organism UniProt Comment Textmining
Elizabethkingia meningoseptica
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dEDANSylated hen ovomucoid glycopeptide + H2O DGP Elizabethkingia meningoseptica ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 34700, native enzyme, 314 amino acid open reading frame Elizabethkingia meningoseptica

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
 Elizabethkingia meningoseptica

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
-
 Elizabethkingia meningoseptica