EC Number   |
Application |
Reference |
|---|
   3.4.24.27 | analysis |
selective biotin tagging and thermolysin proteolysis of chloroplast outer envelope proteins reveals information on protein topology and association into complexes. Development and evaluation of a method providing information at the surface of the outer envelope membrane, based on specific tagging with biotin or proteolysis using thermolysin, a non-membrane permeable protease. Envelope, thylakoid, and stroma proteins are separated by two-dimensional electrophoresis and analyzed by immunostaining and mass spectrometry, overview |
733917 |
| 3.4.24.27 | analysis |
thermolysin degrades cellular prion protein while preserving both proteinase K-sensitive and proteinase K-resistant isoforms of disease-related prion protein in both rodent and human prion strains. In variant Creutzfeldt-Jakob disease, up to 90% of total prion protein present in the brain resists degradation with thermolysin, whereas only about 15% of this material resists digestion by proteinase K |
696101 |
| 3.4.24.27 | analysis |
thermolysin offers a tool for complete solubilization of cartilage prior to comprehensive glycosaminoglycans(GAG)omic analysis, and is likely applicable to other collagen-rich tissues such as ligaments, skin, and blood vessels |
752473 |
| 3.4.24.27 | diagnostics |
thermolysin is used in the diagnosis of prion diseases ovine scrapie and bovine spongiform encephalopathy, with similar sensitivity compared to proteinase K digestion, use of a protease to distinguish PrPC from PrPSc, overview |
683862 |
| 3.4.24.27 | food industry |
the enzyme can be used for production of caseicin A, an antimicrobial active peptide, from alpha-casein, for potential improvement of the safety of infant milk formula using milk-derived bioactive peptides |
733978 |
| 3.4.24.27 | industry |
the enzyme is used for synthesis of N-carbobenzyloxy L-Asp-L-Phe methyl ester, a precursor of the artificial sweetener aspartam |
-, 683276 |
| 3.4.24.27 | medicine |
thermolysin degrades cellular prion protein while preserving both proteinase K-sensitive and proteinase K-resistant isoforms of disease-related prion protein in both rodent and human prion strains. In variant Creutzfeldt-Jakob disease, up to 90% of total prion protein present in the brain resists degradation with thermolysin, whereas only about 15% of this material resists digestion by proteinase K |
696101 |
| 3.4.24.27 | more |
thermolysin crystal can be used as the stationary phase in liquid chromatography to separate D/L-phenylglycine, thermolysin crystal is useful for chiral separation |
712856 |
| 3.4.24.27 | more |
thermolysin powder is not acutely toxic with an oral LD50 of more than 18,000 mg/kg (2520 mg/kg thermolysin protein) in rats and more than 24,000 mg/kg (3360 mg/kg protein) in mice. Subchronic feeding studies in rats for 91 days at doses up to 1000 mg/kg (390 mg/kg protein) revealed no significant differences between treated and non-treated groups, no evidence from allergenicity sequence analysis that thermolysin is an allergen, thermolysin is safe for use in food production |
733899 |
| 3.4.24.27 | more |
thermolysin treatment improves the yield of human intestinal epithelial cells. The thermolysin and endothelin-3 method can be used to isolate and generate viable human intestinal epithelial cells from the human small intestine |
711647 |
