EC Number   |
Application |
Reference |
|---|
    2.4.2.3 | diagnostics |
UPP1 may serve as a negative prognosticator in glioma, analysis of the prognostic value of UPP1 for glioma, overview. UPP1 predicts shorter survival for glioma |
758991 |
| 2.4.2.3 | diagnostics |
upregulation of UPP1 increases lymph node metastasis risk and is useful as diagnostic, prognostic, and predictive biomarker in thyroid cancer |
759543 |
| 2.4.2.3 | drug development |
UP12 is a target for development of enzyme inhibitors for cancer chemotherapy, design of inhibitors is intended to boost endogenous uridine levels to rescue normal tissues from the toxicity of fluoropyrimidine nucleoside chemotherapeutic agents, such as capecitabine and 5-fluorouracil. |
701939 |
| 2.4.2.3 | medicine |
the paired expression level of the uridine phosphorylase gene in gastric cancer is a possible prognostic marker for patients with 5-fluorouracil treatment |
684470 |
| 2.4.2.3 | medicine |
uridine phosphorylase plays an important role in the antineoplastic activity of 5-fluorouracil and in the anabolism of its oral prodrug, capecitabine, through the conversion of 5'-deoxy-5-fluorouridineinto 5-fluorouracil. TNF-alpha efficiently induces UPase gene expression through a NF-kappaB subunit p65-dependent pathway enhancing cell sensitivity to 5'-deoxy-5-fluorouridine. The elucidation of this regulation mechanism may aid in the clinical use of 5-fluorouracil-based chemotherapy |
676094 |
| 2.4.2.3 | synthesis |
evolvement of a mutant enzyme by iterative saturation mutagenesis. Compared to the wild type enzyme, which has a temperature optimum of 40°C and a half-life of 9.89 h at 60°C, the selected mutant has a temperature optimum of 60°C and a half-life of 17.3 h at 60°C. Self-immobilization of the native enzyme as a Spherezyme shows a 3.3fold increase in thermostability while immobilized mutant enzyme shows a 4.4fold increase in thermostability. Combining the enzyme with the purine nucleoside phosphorylase from Bacillus halodurans allows for synthesis of 5-methyluridine (a pharmaceutical intermediate) from guanosine and thymine in a one-pot transglycosylation reaction. Replacing the wild type uridine phosphorylase with the mutant allows for an increase in reaction temperature to 65°C and increased the reaction productivity from 10 to 31 g per l and h |
723000 |
| 2.4.2.3 | synthesis |
the enzyme is a valuable industrial biocatalysts for high-temperature reactions that produce nucleoside drugs in high yields |
-, 726781 |
| 2.4.2.3 | synthesis |
two-step efficient synthesis of 5-methyluridine, a intermediate in the synthesis of anti-AIDS drug such as stavudine and zidovudine |
721810 |
