Application | Comment | Organism |
---|---|---|
drug development | UP12 is a target for development of enzyme inhibitors for cancer chemotherapy, design of inhibitors is intended to boost endogenous uridine levels to rescue normal tissues from the toxicity of fluoropyrimidine nucleoside chemotherapeutic agents, such as capecitabine and 5-fluorouracil. | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
expression of UP1 in Escherichia coli strain Rosetta (DE3) | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
alpha-D-ribose 1-phosphate | product inhibition pattern, noncompetitive versus uridine, competitive versus phosphate, overview | Homo sapiens | |
Uracil | product inhibition pattern, noncompetitive versus uridine and phosphate, overview | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | steady-state kinetics | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
uridine + phosphate | Homo sapiens | - |
uracil + alpha-D-ribose 1-phosphate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q16831 | UP1; type 1 enzyme | - |
Purification (Comment) | Organism |
---|---|
recombinant UP1 3.8fold from Escherichia coli strain Rosetta (DE3) | Homo sapiens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
7.7 | - |
purified recombinant enzyme | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
uridine + phosphate | - |
Homo sapiens | uracil + alpha-D-ribose 1-phosphate | - |
r |
Synonyms | Comment | Organism |
---|---|---|
UP type 1 | - |
Homo sapiens |
UP1 | - |
Homo sapiens |
uridine phosphorylase 1 | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.6 | - |
recombinant enzyme | Homo sapiens |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 9 | recombinant enzyme,m pH profile, overview | Homo sapiens |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.034 | - |
alpha-D-ribose 1-phosphate | pH 7.4, 37°C, versus uridine | Homo sapiens | |
0.05 | - |
Uracil | pH 7.4, 37°C, versus uridine | Homo sapiens | |
0.137 | - |
Uracil | pH 7.4, 37°C, versus phosphate | Homo sapiens | |
0.494 | - |
alpha-D-ribose 1-phosphate | pH 7.4, 37°C, versus phosphate | Homo sapiens |
General Information | Comment | Organism |
---|---|---|
metabolism | uridine phosphorylase is a key enzyme in the pyrimidine salvage pathway, catalyzing the reversible phosphorolysis of uridine to uracil and ribose-1-phosphate | Homo sapiens |