EC Number |
Organic Solvent |
Reference |
---|
3.1.8.1 | guanidine-HCl |
the conformational stability of SsoPox against the denaturing action of guanidine-HCl has been investigated at 25°C, pH 8.0, 20 mM TrisHCl buffer, by performing CD and fluorescence measurements. The transition curves obtained by recording the molar ellipticity at 222 nm (detecting the secondary structure stability) present two inflection points, at 2.6 M guanidine-HCl and about 4.8 M guanidine-HCl, respectively, with a plateau at about 3 M guanidine-HCl. SsoPox is markedly more resistant to the denaturing action of guanidine-HCl with respect to the mesophilic counterpart. Overall guanidine-HCl-induced denaturation of SsoPox is not a reversible process in all the investigated experimental conditions: upon suitable dilution of fully denatured samples, there is not a complete recovery of the far-UV CD spectrum or fluorescence emission spectrum of the native enzyme |
-, 719426 |
3.1.8.1 | more |
the enzyme activity is not affected by acetone, acetonitrile, butanone, butyl acetate, chloroform, dichloromethane, diethyl ether, ethanol, ethyl acetate, isopropanol, methanol, and methoxypropanol |
752208 |
3.1.8.1 | Triton X-100 |
after incubation for 60 min with Triton X-100 the enzyme activity is remarkably decreased with increasing temperature and concentration |
-, 692889 |
3.1.8.1 | urea |
the purified enzyme shows high stability against urea |
-, 692889 |