EC Number   |
Organic Solvent |
Reference |
|---|
    3.1.1.1 | 1,4-dioxane |
30%, about 60% inhibition, at 70°C, in 50 mM HEPES (pH 7.5) using 4-nitrophenyl butanoate as the substrate |
-, 679887 |
| 3.1.1.1 | 1,4-dioxane |
the enzyme activity is reduced by 60-95.5% at 5-10% v/v |
749622 |
| 3.1.1.1 | 1-butanol |
30% v/v, recombinant enzyme expressed in Sulfolobus solfataricus shows 36% residual activity. The recombinant enzyme expressed in Escherichia coli as thioredoxin-free form (EcSisEstA I) shows 18% residual activity. The recombinant enzyme expressed in Escherichia coli as a thioredoxin-EstA fusion protein (EcSisEstA II) shows 29% residual activity |
724065 |
| 3.1.1.1 | 1-propanol |
30% v/v, recombinant enzyme expressed in Sulfolobus solfataricus shows 76% residual activity. The recombinant enzyme expressed in Escherichia coli as thioredoxin-free form (EcSisEstA I) shows 32% residual activity. The recombinant enzyme expressed in Escherichia coli as a thioredoxin-EstA fusion protein (EcSisEstA II) shows 39% residual activity |
724065 |
| 3.1.1.1 | 2-propanol |
1 h, 80%, 6% loss of activity |
-, 726707 |
| 3.1.1.1 | 2-propanol |
30% v/v, recombinant enzyme expressed in Sulfolobus solfataricus shows 95% residual activity. The recombinant enzyme expressed in Escherichia coli as thioredoxin-free form (EcSisEstA I) shows 30% residual activity. The recombinant enzyme expressed in Escherichia coli as a thioredoxin-EstA fusion protein (EcSisEstA II) shows 35% residual activity |
724065 |
| 3.1.1.1 | 2-propanol |
40%, 60 min at 30°C, 91% residual activity |
-, 678386 |
| 3.1.1.1 | 2-propanol |
90%, 30°C, 30 min, stable |
-, 725745 |
| 3.1.1.1 | Acetone |
15% v/v, 128% of initial activity, 30% v/v, 155% of initial activity |
677663 |
| 3.1.1.1 | Acetone |
15% v/v, 14% of initial activity |
677663 |
