EC Number |
Molecular Weight [Da] |
Molecular Weight Maximum [Da] |
Reference |
---|
6.5.1.1 | -999 |
- |
DNA ligase I, and II have a markedly asymetric structure, gel filtration data lead to a gross overestimation of the MW |
1906 |
6.5.1.1 | 31000 |
32000 |
analytical ultracentrifugation |
650253 |
6.5.1.1 | 31540 |
- |
gel filtration after preincubation with Mg2+ and diphosphate |
650253 |
6.5.1.1 | 31548 |
- |
1 * 31548, deduced from nucleotide sequence |
650253 |
6.5.1.1 | 31873 |
- |
1 * 31873, mass spectroscopy |
650253 |
6.5.1.1 | 41000 |
- |
- |
652785 |
6.5.1.1 | 41000 |
- |
equilibrium sedimentation |
1946 |
6.5.1.1 | 44000 |
- |
x * 44000, DNA ligase V, SDS-PAGE |
1938 |
6.5.1.1 | 46000 |
- |
1 * 46000 + 1 * 100000, DNA ligase III, SDS-PAGE. It cannot be concluded whether the 46000 MW peptide is an essential and integral part of DNA ligase III, or if it represents a separate protein that binds tentaciously to the 100000 MW DNA ligase component |
1906 |
6.5.1.1 | 52100 |
- |
gel filtration |
651669 |