1.1.1.2 | -999 |
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kinetic analysis of wild-type and mutant enzymes, fit to the ordered bi-bi rate equation, steady-state kinetics, detailed overview. For some mutants (G211S, G211C, G211InsA and G211InsS), with NAD+ as a cofactor, the KA or KB values are observed to be higher than the maximum concentration of substrate or cofactor utilized. So, for mutants G211S, G211C, G211InsA and G211InsS, kinetic data is also measured using assay reaction mixtures containing 1-450 mM 2,3-butanediol and 0.001-5.50 mM NAD+ |
746361 |