EC Number |
General Stability |
Reference |
---|
2.5.1.21 | a combination of 10% methanol, 10% glycerol, 30 mM octyl-beta-D-glucopyranoside, 0.4% Brij-58, and 1 mM DTT in 25 mM sodium phosphate, pH 7.4, essential for stability and maximal activity |
489839 |
2.5.1.21 | detergent, e.g. deoxycholate, destabilizes |
489836 |
2.5.1.21 | markedly stabilized by 30% glycerol and 0.02 M 2-mercaptoethanol |
489843 |
2.5.1.21 | methanol stabilizes |
489826 |
2.5.1.21 | methanol, 10%, does not stabilize |
489835, 489838 |
2.5.1.21 | NADPH has no effect on stability |
489846 |
2.5.1.21 | octylglucoside, 5 mM, causes rapid loss of activity |
489838 |
2.5.1.21 | stable in buffer containing 5 mM octylglucoside and 15 mM CHAPS |
489838 |
2.5.1.21 | sucrose stabilizes |
489826 |
2.5.1.21 | sucrose, 10%, does not stabilize |
489835, 489838 |